The mouse lymphoma L5178Y-3.7.2C (TK+/-) cell line is extensively used in genetic toxicology to conduct the mouse lymphoma assay (MLA). The MLA is used to establish the mutagenic and clastogenic effects of chemicals and pharmaceuticals, and is one of the few genetic tests widely accepted by regulatory agencies throughout the world. Despite the extensive use and regulatory impact of L5178Y-3.7.2C (TK+/-) cells, little is known about their genetic composition or how it affects the outcome of the MLA. To determine the genetic background of this cell line, we sequenced and analyzed its entire genome. Our results confirm the existence of previously described mutations in the Tk1 and Trp53 genes and catalog millions of other mutations, many of which impair the function of genes with key roles in cell physiology and genetic toxicology.

中文翻译：

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小鼠淋巴瘤 L5178Y-3.7.2C (TK+/-) 的全基因组测序揭示了数百万个突变和遗传标记。

小鼠淋巴瘤 L5178Y-3.7.2C (TK+/-) 细胞系广泛用于遗传毒理学以进行小鼠淋巴瘤检测 (MLA)。MLA 用于确定化学品和药物的致突变和致畸作用，是为数不多的被全球监管机构广泛接受的基因测试之一。尽管 L5178Y-3.7.2C (TK+/-) 细胞的广泛使用和监管影响，但对其遗传组成或它如何影响 MLA 结果知之甚少。为了确定该细胞系的遗传背景，我们对其整个基因组进行了测序和分析。我们的结果证实了先前描述的 Tk1 和 Trp53 基因突变的存在，并记录了数百万个其他突变，其中许多会损害在细胞生理学和遗传毒理学中起关键作用的基因的功能。