Ganoderma lucidum total triterpenes were evaluated for its apoptosis-inducing and anti-cancer activities. Cytotoxicity and pro-apoptotic effect of total triterpenes were evaluated in human breast adenocarcinoma (MCF-7) cell line using MTT assay and DNA fragmentation analysis. Total triterpenes induced apoptosis in MCF-7 cells by down-regulating the levels of cyclin D1, Bcl-2, Bcl-xL and also by up-regulating the levels of Bax and caspase-9. Anti-carcinogenicity of total triterpenes was analysed using dimethyl benz [a] anthracene (DMBA) induced skin papilloma and mammary adenocarcinoma in Swiss albino mice and Wistar rats respectively. Topical application of 5mg, 10mg and 20mg total triterpenes reduced the incidence of skin papilloma by 62.5, 37.5 and 12.5% respectively. Incidence of the mammary tumour was also reduced significantly by 33.33, 66.67 and 16.67% in 10, 50 and 100mg/kg b.wt. total triterpenes treated animals respectively. Total triterpenes were also found to reduce the average number of tumours per animal and extended the tumour latency period in both the models. The results indicate the potential cytotoxicity and anti-cancerous activity of total triterpenes, there by opens up a path to the development of a safe and successive chemo preventive agent of natural origin.

中文翻译：

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灵芝总三萜在 MCF-7 细胞中诱导细胞凋亡，并在实验动物中减轻 DMBA 诱导的乳腺癌和皮肤癌。

评估了灵芝总三萜的诱导凋亡和抗癌活性。使用 MTT 测定和 DNA 片段分析在人乳腺癌 (MCF-7) 细胞系中评估总三萜的细胞毒性和促凋亡作用。总三萜通过下调细胞周期蛋白 D1、Bcl-2、Bcl-xL 的水平以及上调 Bax 和 caspase-9 的水平来诱导 MCF-7 细胞凋亡。使用二甲基苯并 [a] 蒽 (DMBA) 分别在瑞士白化小鼠和 Wistar 大鼠中诱导皮肤乳头状瘤和乳腺腺癌，分析总三萜的抗癌性。5mg、10mg和20mg总三萜类的局部应用使皮肤乳头状瘤的发生率分别降低了62.5%、37.5%和12.5%。乳腺肿瘤的发生率也显着降低了 33.33、66。在 10、50 和 100mg/kg b.wt. 中分别为 67% 和 16.67%。总三萜分别处理动物。还发现总三萜减少了每只动物的平均肿瘤数量并延长了两种模型中的肿瘤潜伏期。结果表明总三萜具有潜在的细胞毒性和抗癌活性，从而为开发安全且连续的天然来源的化学预防剂开辟了道路。