Increased methylation of repetitive elements and DNA repair genes is associated with higher DNA oxidation in children in an urbanized, industrial environment.,Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

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Increased methylation of repetitive elements and DNA repair genes is associated with higher DNA oxidation in children in an urbanized, industrial environment.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Pub Date : 2017 Jan , DOI: 10.1016/j.mrgentox.2016.11.007
Isabel Alvarado-Cruz ₁ , Marco Sánchez-Guerra ₁ , Leticia Hernández-Cadena ₂ , Andrea De Vizcaya-Ruiz ₁ , Violeta Mugica ₃ , Nadia Azenet Pelallo-Martínez ₁ , María de Jesús Solís-Heredia ₁ , Hyang-Min Byun ₄ , Andrea Baccarelli ₄ , Betzabet Quintanilla-Vega ₁

Affiliation

DNA methylation in DNA repair genes participates in the DNA damage regulation. Particulate matter (PM), which has metals and polycyclic aromatic hydrocarbons (PAHs) adsorbed, among others has been linked to adverse health outcomes and may modify DNA methylation. To evaluate PM exposure impact on repetitive elements and gene-specific DNA methylation and DNA damage, we conducted a cross-sectional study in 150 schoolchildren (7-10 years old) from an urbanized, industrial area of the metropolitan area of Mexico City (MAMC), which frequently exhibits PM concentrations above safety standards. Methylation (5mC) of long interspersed nuclear element-1 (LINE1) and DNA repair gene (OGG1, APEX, and PARP1) was assessed by pyrosequencing in peripheral mononuclear cells, DNA damage by comet assay and DNA oxidation by 8-OHdG content. PAH and metal contents in PM10 (

中文翻译：

重复元件和 DNA 修复基因的甲基化增加与城市化工业环境中儿童的 DNA 氧化程度更高有关。

DNA 修复基因中的 DNA 甲基化参与 DNA 损伤调节。吸附了金属和多环芳烃 (PAH) 等的颗粒物 (PM) 与不良健康结果有关，并可能改变 DNA 甲基化。为了评估 PM 暴露对重复元素和基因特异性 DNA 甲基化和 DNA 损伤的影响，我们对来自墨西哥城大都市区 (MAMC) 的城市化工业区的 150 名学童（7-10 岁）进行了横断面研究。 )，这经常表现出高于安全标准的 PM 浓度。长散在核元件 1 (LINE1) 和 DNA 修复基因 (OGG1、APEX 和 PARP1) 的甲基化 (5mC) 通过外周单核细胞中的焦磷酸测序、彗星测定的 DNA 损伤和 8-OHdG 含量的 DNA 氧化来评估。

更新日期：2017-01-31

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