Both ionizing radiation (IR) and psychological stress (PS) cause detrimental effects on humans. A recent study showed that chronic restraint-induced PS (CRIPS) diminished the functions of Trp53 and enhanced radiocarcinogenesis in Trp53-heterozygous (Trp53+/-) mice. These findings had a marked impact on the academic field as well as the general public, particularly among residents living in areas radioactively contaminated by nuclear accidents. In an attempt to elucidate the modifying effects of CRIPS on radiation-induced health consequences in Trp53 wild-type (Trp53+/+) animals, investigations involving multidisciplinary analyses were performed. We herein demonstrated that CRIPS induced changes in the frequency of IR-induced chromosomal aberrations (CAs) in splenocytes. Five-week-old male Trp53+/+ C57BL/6J mice were restrained for 6h per day for 28 consecutive days, and total body irradiation (TBI) at a dose of 4Gy was performed on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with fluorescence in situ hybridization (FISH) probes for chromosomes 1, 2, and 3. The results obtained showed that CRIPS alone did not induce CAs, while TBI caused significant increases in CAs, mostly translocations. Translocations appeared at a lower frequency in mice exposed to TBI plus CRIPS than in those exposed to TBI alone. No significant differences were observed in the frequencies of the other types of CAs (insertions, dicentrics, and fragments) visualized with FISH between these experimental groups (TBI+CRIPS vs. TBI). These results suggest that CRIPS does not appear to synergize with the clastogenicity of IR.

中文翻译：

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慢性束缚诱导应激对小鼠脾细胞辐射诱导染色体畸变的影响。

电离辐射 (IR) 和心理压力 (PS) 都会对人类造成不利影响。最近的一项研究表明，慢性束缚诱导的 PS (CRIPS) 减弱了 Trp53 的功能并增强了 Trp53 杂合 (Trp53+/-) 小鼠的放射性致癌作用。这些发现对学术界和公众产生了显着影响，尤其是生活在核事故放射性污染地区的居民。为了阐明 CRIPS 对 Trp53 野生型 (Trp53+/+) 动物辐射诱导的健康后果的改变作用，进行了涉及多学科分析的调查。我们在此证明 CRIPS 诱导脾细胞中 IR 诱导的染色体畸变 (CA) 的频率发生变化。5 周龄雄性 Trp53+/+ C57BL/6J 小鼠连续 28 天每天约束 6 小时，第 8 天进行 4Gy 剂量的全身照射（TBI）。在 28 天约束方案结束时从脾细胞制备的中期染色体涂片涂有针对染色体 1、2 和 3 的荧光原位杂交 (FISH) 探针。获得的结果表明，单独使用 CRIPS 不会诱导 CA，而 TBI导致 CA 显着增加，主要是易位。与仅暴露于 TBI 的小鼠相比，暴露于 TBI 加 CRIPS 的小鼠的易位频率较低。在这些实验组（TBI+CRIPS 与 TBI）之间用 FISH 可视化的其他类型 CA（插入物、双着丝粒和碎片）的频率没有观察到显着差异。