The brown planthopper (BPH) and white-backed planthopper (WBPH) are the most destructive insect pests of rice, and they pose serious threats to rice production throughout Asia. Thus, there are urgent needs to identify resistance-conferring genes and to breed planthopper-resistant rice varieties. Here we report the map-based cloning and functional analysis of Bph6, a gene that confers resistance to planthoppers in rice. Bph6 encodes a previously uncharacterized protein that localizes to exocysts and interacts with the exocyst subunit OsEXO70E1. Bph6 expression increases exocytosis and participates in cell wall maintenance and reinforcement. A coordinated cytokinin, salicylic acid and jasmonic acid signaling pathway is activated in Bph6-carrying plants, which display broad resistance to all tested BPH biotypes and to WBPH without sacrificing yield, as these plants were found to maintain a high level of performance in a field that was heavily infested with BPH. Our results suggest that a superior resistance gene that evolved long ago in a region where planthoppers are found year round could be very valuable for controlling agricultural insect pests.

中文翻译：

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Bph6编码一种囊外定位蛋白，赋予水稻稻飞虱广泛的抗性。

褐飞虱（BPH）和白背飞虱（WBPH）是稻米最具破坏性的害虫，它们对整个亚洲的稻米生产构成严重威胁。因此，迫切需要鉴定赋予抗性的基因并育成对飞虱具有抗性的水稻品种。在这里，我们报告基于Bph6的基于图的克隆和功能分析，该基因赋予水稻稻飞虱抗性。Bph6编码一个以前未表征的蛋白质，该蛋白质定位在囊外并与囊外亚基OsEXO70E1相互作用。Bph6表达增加胞吐作用并参与细胞壁的维持和增强。在携带Bph6的植物中激活了协调的细胞分裂素，水杨酸和茉莉酸信号通路，该植物对所有测试的BPH生物型和WBPH具有广泛的抗性，而不会牺牲产量，因为这些植物被发现在充满BPH的田地中保持较高的性能。我们的研究结果表明，一个很长的抗性基因很早以前就在一年四季都发现有飞虱的地区进化出，对于控制农业害虫可能是非常有价值的。