Abstract Nano-bio interactions are dynamic, interrelated, and overwhelmingly influence nanomedicine efficacy. Molecular imaging techniques such as fluorescence are essential for illuminating the nano-bio interface to guide nanomedicine development. With advances in reliable labelling of nanoparticles with fluorophores, integrated Forster resonance energy transfer (FRET) provides responsive fluorescence linked to nanomedicine integrity and function. In this review, we provide an overview of developments in the application of integrated FRET for imaging nanomedicines during nano-bio interactions. Furthermore, we discuss technical considerations and a general methodology for maximizing information from integrated FRET imaging and advancing its use as a routine tool in nanomedicine research.

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FRET成像指导的纳米医学发展

摘要 纳米生物相互作用是动态的、相互关联的，并且极大地影响纳米医学的功效。荧光等分子成像技术对于照亮纳米生物界面以指导纳米医学发展至关重要。随着用荧光团对纳米粒子进行可靠标记的进展，集成的 Forster 共振能量转移 (FRET) 提供了与纳米医学完整性和功能相关的响应荧光。在这篇综述中，我们概述了集成 FRET 在纳米生物相互作用过程中对纳米医学成像的应用进展。此外，我们讨论了从集成 FRET 成像中最大化信息并将其用作纳米医学研究中的常规工具的技术考虑因素和一般方法。