SUMMARY The use of lentiviral vectors for therapeutic purposes has shown promising results in clinical trials. The ability to produce a clinical-grade vector at high yields remains a critical issue. One possible obstacle could be cellular factors known to inhibit human immunodeficiency virus (HIV). To date, five HIV restriction factors have been identified, although it is likely that more factors are involved in the complex HIV-cell interaction. Inhibitory factors that have an adverse effect but do not abolish virus production are much less well described. Therefore, a gap exists in the knowledge of inhibitory factors acting late in the HIV life cycle (from transcription to infection of a new cell), which are relevant to the lentiviral vector production process. The objective was to review the HIV literature to identify cellular factors previously implicated as inhibitors of the late stages of lentivirus production. A search for publications was conducted on MEDLINE via the PubMed interface, using the keyword sequence “HIV restriction factor” or “HIV restriction” or “inhibit HIV” or “repress HIV” or “restrict HIV” or “suppress HIV” or “block HIV,” with a publication date up to 31 December 2016. Cited papers from the identified records were investigated, and additional database searches were performed. A total of 260 candidate inhibitory factors were identified. These factors have been identified in the literature as having a negative impact on HIV replication. This study identified hundreds of candidate inhibitory factors for which the impact of modulating their expression in lentiviral vector production could be beneficial.

概括慢病毒载体用于治疗目的已在临床试验中显示出令人鼓舞的结果。以高产量产生临床级载体的能力仍然是一个关键问题。一个可能的障碍可能是已知抑制人类免疫缺陷病毒（HIV）的细胞因子。迄今为止，已经确定了五个HIV限制因素，尽管可能有更多因素参与了复杂的HIV细胞相互作用。对具有不利影响但不会消除病毒产生的抑制因子的描述要少得多。因此，在抑制因子在HIV生命周期后期（从转录到新细胞的感染）起作用的知识方面存在差距，这与慢病毒载体的生产过程有关。目的是审查HIV文献，以鉴定以前与慢病毒生产后期抑制剂有关的细胞因子。通过PubMed界面在MEDLINE上搜索出版物，使用关键词序列“ HIV限制因子”或“ HIV限制”或“抑制HIV”或“抑制HIV”或“限制HIV”或“抑制HIV”或“阻断” 《艾滋病毒》，出版日期至2016年12月31日。对已查明记录的被引论文进行了调查，并进行了其他数据库搜索。总共鉴定了260种候选抑制因子。这些因素已在文献中确定为对HIV复制具有负面影响。