Some β-glucans have attracted attention due to their functionality as an immunostimulant and have been used in processed foods. However, accurately measuring the β-glucan content of processed foods using existing methods is difficult. We demonstrate a new method, the Sodium hypochlorite Extracting and Enzymatic Digesting (SEED) assay, in which β-glucan is extracted using sodium hypochlorite, dimethyl sulfoxide, and 5 mol/L sodium hydroxide and then digested into β-glucan fragments using Westase which is an enzyme having β-1,6- and β-1,3 glucanase activity. The β-glucan fragments are further digested into glucose using exo-1,3-β-d-glucanase and β-glucosidase. We measured β-glucan comprising β-1,3-, -1,6-, and -1,(3),4- bonds in various polysaccharide reagents and processed foods using our novel method. The SEED assay was able to quantify β-glucan with good reproducibility, and the recovery rate was >90% for food containing β-glucan. Therefore, the SEED assay is capable of accurately measuring the β-glucan content of processed foods.

中文翻译：

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定量加工食品中β-葡聚糖的新方法：次氯酸钠提取和酶消化（SEED）分析

一些β-葡聚糖由于其作为免疫刺激剂的功能而备受关注，并已用于加工食品中。然而，使用现有方法准确地测量加工食品中的β-葡聚糖含量是困难的。我们演示了一种新的方法-次氯酸钠提取和酶促消化（SEED）分析，其中使用次氯酸钠，二甲基亚砜和5 mol / L氢氧化钠提取β-葡聚糖，然后使用Westase将其消化成β-葡聚糖片段，是具有β-1，6-和β-1，3葡聚糖酶活性的酶。使用exo-1,3-β- d将β-葡聚糖片段进一步消化成葡萄糖-葡聚糖酶和β-葡糖苷酶。使用我们的新方法，我们在各种多糖试剂和加工食品中测量了包含β-1,3-，-1,6-和-1，（3），4-键的β-葡聚糖。SEED分析能够以良好的可重复性对β-葡聚糖进行定量，对于含β-葡聚糖的食品，其回收率> 90％。因此，SEED分析能够准确地测量加工食品中的β-葡聚糖含量。