Razor clam is a major cultivated shellfish of great economic importance and high nutritional value. Due to high corruptible potential, razor clam is generally preserved by controlled freezing-point storage (CFPS). Here, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling to investigate the biochemical mechanism of cold stress adaption in razor clam during CFPS. In total, 369 proteins were quantified, and 27 of them were identified as differentially expressed proteins during CFPS, mostly involved in energy metabolism process, DNA duplication and protein synthesis, and stress response, specifically, MAPK is the predominant pathway. Further qPCR results revealed H2A and S6K 2 alpha to be the critical post-transcriptionally regulated genes. Our results provided proteomics information with respect to the biochemical mechanism of cold stress adaption in razor clam, shed light on the further elongation of razor clams storage period, and help clarify the novel mechanisms of cold tolerance.

剃刀蛤是具有重要经济意义和高营养价值的主要养殖贝类。由于极高的潜在破坏力，通常通过控制冰点存储（CFPS）来保存剃刀蛤。在这里，我们应用等压标记进行相对和绝对定量（iTRAQ）标记，以研究CFPS期间剃刀蛤中冷应激适应的生化机制。总共定量了369种蛋白质，其中27种被鉴定为CFPS期间差异表达的蛋白质，主要涉及能量代谢过程，DNA复制和蛋白质合成以及应激反应，特别是MAPK是主要途径。进一步的qPCR结果显示H2A和S6K 2 alpha是转录后调控的关键基因。