Benzodiazepines are often used for the treatment of epilepsy, convulsions, and many psychiatric disorders. The widespread use of this class of drugs has occasionally raised concern about recreational benzodiazepine abuse and has led to the erroneous impression that benzodiazepines have a relatively high abuse liability among recreational drug users. Therefore, the separation and identification of these compounds is of great interest. In general, the separation of benzodiazepines is performed using high-performance liquid chromatography (HPLC). Recently, capillary electrochromatography, which combines the high efficiency of capillary zone electrophoresis and the high selectivity of HPLC, has gained much attention. The focus of the work reported here is the use of a 40-cm packed bed of Reliasil 3- microm C(18) stationary phase to separate seven benzodiazepines. Optimal conditions are established by varying the mobile phase, amount of organic modifier, buffer concentration, applied voltage, and column temperature. A mobile phase composition of Tris-HCl (pH 8)-acetonitrile (60:40), an electrolyte concentration of 30mM, and a temperature of 15 degrees C with an applied voltage of 20 kV proves to be optimum. In addition, the method developed here is applied to the characterization of oxazepam in a standard urine sample.

中文翻译：

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使用毛细管电色谱法分离苯二氮卓类药物。

苯二氮卓类药物通常用于治疗癫痫，惊厥和许多精神疾病。这类药物的广泛使用偶尔引起人们对休闲性苯二氮卓类药物滥用的关注，并导致错误的印象，即休闲性吸毒者中苯二氮卓类药物具有较高的滥用责任。因此，这些化合物的分离和鉴定是非常令人感兴趣的。通常，苯并二氮杂separation的分离是使用高效液相色谱法（HPLC）进行的。近年来，毛细管电色谱结合了毛细管区带电泳的高效率和HPLC的高选择性，备受关注。这里报道的工作重点是使用Reliasil 3微米C（18）固定相的40厘米填充床分离七个苯并二氮杂pine。通过改变流动相，有机改性剂的量，缓冲液浓度，施加的电压和色谱柱温度来确定最佳条件。事实证明，使用Tris-HCl（pH 8）-乙腈（60:40），电解质浓度为30mM，温度为15摄氏度，施加电压为20 kV的流动相是最佳的。此外，此处开发的方法适用于标准尿液样品中奥沙西m的表征。电解质浓度为30mM，施加电压为20 kV的温度为15摄氏度时被证明是最佳的。此外，此处开发的方法适用于标准尿液样品中奥沙西m的表征。电解质浓度为30mM，施加温度为20 kV的温度为15摄氏度时，被证明是最佳的。此外，此处开发的方法适用于标准尿液样品中奥沙西m的表征。