This work describes the synchronized dual-polarity (DP) electrospray ionization (ESI) method and demonstrates the first DP ESI mass spectra obtained using two mass spectrometers. Stable double Taylor cones were produced by applying two counter electric voltages with opposite polarities to one electrosprayer. The development of double Taylor cones required higher extraction voltages than conventional ESI, but DP ESI worked effectively at liquid flow rate range three times wider than conventional ESI. Using pure methanol, the emission currents of the two cones were neutralized and no current was drawn from the sprayer. Synchronized DP mass spectra were obtained using electrospray calibrants dissolved in methanol solution of low water content. For bovine insulin with conventional electrospray solution, the gas-assisted electrospray delivered satisfactory sensitivity and stability for routine mass analyses.

这项工作描述了同步双极性 (DP) 电喷雾电离 (ESI) 方法，并展示了使用两个质谱仪获得的第一个 DP ESI 质谱。通过向一个电喷雾器施加两个极性相反的反电压来产生稳定的双泰勒锥。双泰勒锥的开发需要比传统 ESI 更高的提取电压，但 DP ESI 在比传统 ESI 宽三倍的液体流速范围内有效工作。使用纯甲醇，两个锥体的发射电流被中和，并且没有电流从喷雾器中汲取。使用溶解在低水含量甲醇溶液中的电喷雾校准物获得同步 DP 质谱。对于使用常规电喷雾溶液的牛胰岛素，