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Polyphasic taxonomy of Aspergillus section Aspergillus (formerly Eurotium), and its occurrence in indoor environments and food
Studies in Mycology ( IF 16.5 ) Pub Date : 2017-07-12 , DOI: 10.1016/j.simyco.2017.07.001
A.J. Chen , V. Hubka , J.C. Frisvad , C.M. Visagie , J. Houbraken , M. Meijer , J. Varga , R. Demirel , Ž. Jurjević , A. Kubátová , F. Sklenář , Y.G. Zhou , R.A. Samson

Aspergillus section Aspergillus (formerly the genus Eurotium) includes xerophilic species with uniseriate conidiophores, globose to subglobose vesicles, green conidia and yellow, thin walled eurotium-like ascomata with hyaline, lenticular ascospores. In the present study, a polyphasic approach using morphological characters, extrolites, physiological characters and phylogeny was applied to investigate the taxonomy of this section. Over 500 strains from various culture collections and new isolates obtained from indoor environments and a wide range of substrates all over the world were identified using calmodulin gene sequencing. Of these, 163 isolates were subjected to molecular phylogenetic analyses using sequences of ITS rDNA, partial β-tubulin (BenA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) genes. Colony characteristics were documented on eight cultivation media, growth parameters at three incubation temperatures were recorded and micromorphology was examined using light microscopy as well as scanning electron microscopy to illustrate and characterize each species. Many specific extrolites were extracted and identified from cultures, including echinulins, epiheveadrides, auroglaucins and anthraquinone bisanthrons, and to be consistent in strains of nearly all species. Other extrolites are species-specific, and thus valuable for identification. Several extrolites show antioxidant effects, which may be nutritionally beneficial in food and beverages. Important mycotoxins in the strict sense, such as sterigmatocystin, aflatoxins, ochratoxins, citrinin were not detected despite previous reports on their production in this section. Adopting a polyphasic approach, 31 species are recognized, including nine new species. ITS is highly conserved in this section and does not distinguish species. All species can be differentiated using CaM or RPB2 sequences. For BenA, Aspergillus brunneus and A. niveoglaucus share identical sequences. Ascospores and conidia morphology, growth rates at different temperatures are most useful characters for phenotypic species identification.



中文翻译:

曲霉部分曲霉(以前称为Euro)的多相分类法及其在室内环境和食物中的发生

曲霉菌部分曲霉菌(以前是Euro属)包括具有不对称分生孢子的嗜干菌种,球状到近球状的囊泡,绿色分生孢子和黄色的薄壁euro状像子,具有透明质,双凸状的子囊孢子。在本研究中,采用形态学特征,外泌体,生理学特征和系统发育的多相方法研究该部分的分类学。使用钙调蛋白基因测序,鉴定了来自各种培养物集合的500多种菌株以及从室内环境获得的新分离株以及世界各地的各种底物。其中的163个分离物使用ITS rDNA,部分β-微管蛋白(BenA),钙调蛋白(CaM)和RNA聚合酶II第二大亚基(RPB2)基因。在八种培养基上记录菌落特征,记录在三个温育温度下的生长参数,并使用光学显微镜和扫描电子显微镜检查微观形态,以说明和表征每个物种。从培养物中提取并鉴定了许多特定的外泌体,包括棘孢菌素,表硬脂酸,金葡聚糖和蒽醌双蒽醌,并且在几乎所有物种的菌株中都一致。其他珍珠岩是特定物种的,因此对于鉴定很有价值。几种珍珠岩显示出抗氧化作用,这可能对食品和饮料的营养有益。尽管以前曾在本节中报道过严格的真菌毒素,如严格的霉菌毒素,黄曲霉毒素,曲霉毒素,citrinin,但仍未检出。采用多相方法,确认了31个物种,其中包括9个新物种。ITS在本节中高度保守,不区分物种。可以使用以下方法区分所有物种CaMRPB2序列。对于贝纳曲霉蠹A. niveoglaucus共享相同的序列。子囊和分生孢子的形态,不同温度下的生长速率是鉴定表型物种最有用的特征。

更新日期:2017-07-12
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