Expert Opinion on Drug Discovery ( IF 6.0 ) Pub Date : 2010-07-15 , DOI: 10.1517/17460441.2010.495116 Raymond Cheong 1 , Saurabh Paliwal , Andre Levchenko
Importance of the field: Miniaturization is the key to advancing the state of the art in high-content screening (HCS) in order to enable dramatic cost savings through reduced usage of expensive biochemical reagents and to enable large-scale screening on primary cells. Microfluidic technology offers the potential to enable HCS to be performed with an unprecedented degree of miniaturization.
Areas covered in this review: This perspective highlights a real-world example from the authors' work of HCS assays implemented in a highly miniaturized microfluidic format. The advantages of this technology are discussed, including cost savings, high-throughput screening on primary cells, improved accuracy, the ability to study complex time-varying stimuli, and ease of automation, integration and scaling.
What the reader will gain: The reader will understand the capabilities of a new microfluidics-based platform for HCS and the advantages it provides over conventional plate-based HCS.
Take home message: Microfluidics technology will drive significant advancements and broader usage and applicability of HCS in drug discovery.
中文翻译:
微流体设备中的高内涵筛选
该领域的重要性:小型化是推进高内涵筛选 (HCS) 技术水平的关键,以便通过减少昂贵生化试剂的使用来显着节省成本,并实现对原代细胞的大规模筛选。微流体技术提供了使 HCS 能够以前所未有的小型化程度执行的潜力。
本评论涵盖的领域:这一观点突出了作者以高度微型化的微流体格式实施的 HCS 检测工作中的一个真实示例。讨论了该技术的优点,包括成本节约、原代细胞的高通量筛选、提高的准确性、研究复杂时变刺激的能力以及自动化、集成和扩展的简易性。
读者将获得什么:读者将了解基于微流体的新 HCS 平台的功能以及它相对于传统的基于板的 HCS 提供的优势。
带回家的信息:微流体技术将推动 HCS 在药物发现中的重大进步和更广泛的使用和适用性。