Open-tubular capillary electrochromatography (OT-CEC) is an alternative approach to conventional CEC. The primary advantage of OT-CEC is the elimination of problems associated with frits and silica particles in conventional CEC. This report is an investigation of the utility of using a polymeric surfactant (molecular micelle) for OT-CEC. In this approach, fused-silica capillaries coated with thin films of physically adsorbed charged polymers are developed by use of a polyelectrolyte multilayer (PEM) coating procedure. The PEM coating is constructed in situ by alternating rinses with positively and negatively charged polymers, where the negatively charged polymer is a molecular micelle. This can offer a number of advantages for separation of hydrophobic analytes. In this study, poly(diallyldimethylammonium chloride) was used as the cationic polymer and poly(sodium N-undecanoyl-L-glycinate) was used as the anionic polymer for PEM coating. The performance of the modified capillaries as a separation medium is evaluated by use of seven benzodiazepines as analytes. The run-to-run, day-to-day, week-to-week, and capillary-to-capillary reproducibilities of electroosmotic flow are very good with relative standard deviation values of less than 1% in all cases. In addition, the chromatographic performance of the monomeric form of the molecular micelle is compared for the separation of these analytes. The PEM-coated capillary was remarkably robust with more than 200 runs accomplished in this study. Strong stability against extreme pH values was also observed. The general utility of this approach is discussed in detail.

中文翻译：

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在开管毛细管电色谱中使用分子胶束进行分析分离。

开管毛细管电色谱法（OT-CEC）是常规CEC的替代方法。OT-CEC的主要优点是消除了传统CEC中与玻璃料和二氧化硅颗粒相关的问题。该报告是对将聚合物表面活性剂（分子胶束）用于OT-CEC的实用性的调查。在这种方法中，通过使用聚电解质多层（PEM）涂层工艺开发了涂覆有物理吸附的带电聚合物薄膜的熔融石英毛细管。通过用带正电和带负电的聚合物交替漂洗原位构建PEM涂层，其中带负电的聚合物是分子胶束。这可以为疏水性分析物的分离提供许多优势。在这项研究中，聚（二烯丙基二甲基氯化铵）用作阳离子聚合物，聚（N-十一烷酰基-L-甘氨酸钠）用作用于PEM涂层的阴离子聚合物。通过使用七种苯二氮卓类化合物作为分析物评估改性毛细管作为分离介质的性能。每次运行，每天，每周，以及毛细管到毛细管的电渗流重现性都非常好，所有情况下的相对标准偏差值均小于1％。此外，比较了分子胶束单体形式的色谱性能，以分离这些分析物。这项研究中完成200多次电泳，PEM涂层的毛细管非常坚固。还观察到了针对极端pH值的强稳定性。详细讨论了这种方法的一般用途。