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Sizing Up Protein–Ligand Complexes: The Rise of Structural Mass Spectrometry Approaches in the Pharmaceutical Sciences
Annual Review of Analytical Chemistry ( IF 5.9 ) Pub Date : 2017-06-12 00:00:00 , DOI: 10.1146/annurev-anchem-061516-045414
Joseph D. Eschweiler 1 , Richard Kerr 1 , Jessica Rabuck-Gibbons 1 , Brandon T. Ruotolo 1
Affiliation  

Capturing the dynamic interplay between proteins and their myriad interaction partners is critically important for advancing our understanding of almost every biochemical process and human disease. The importance of this general area has spawned many measurement methods capable of assaying such protein complexes, and the mass spectrometry–based structural biology methods described in this review form an important part of that analytical arsenal. Here, we survey the basic principles of such measurements, cover recent applications of the technology that have focused on protein–small-molecule complexes, and discuss the bright future awaiting this group of technologies.

中文翻译:


调整蛋白质-配体复合物的大小:药物科学中结构质谱方法的兴起

捕获蛋白质及其无数相互作用伙伴之间的动态相互作用对于增进我们对几乎每个生化过程和人类疾病的理解至关重要。该领域的重要性催生了许多能够测定此类蛋白质复合物的测量方法,而本综述中所述的基于质谱的结构生物学方法是该分析武库的重要组成部分。在这里,我们调查了此类测量的基本原理,涵盖了专注于蛋白质-小分子复合物的技术的最新应用,并讨论了等待此类技术的光明前景。

更新日期:2017-06-12
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