Chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) is a common and serious disease with unclear pathogenesis and recurrent symptoms. Hedyotis diffusa Willd (HDW) has been recognized for its potential in managing various chronic inflammatory diseases. This research aimed to interrogate the mechanism of HDW in treating CP/CPPS. Complete Freund Adjuvant (CFA) and LPS were utilized to establish the rat and cell models of CP/CPPS. Results showed that HDW decreased levels of inflammation‐related factors in CP rat prostate tissue and LPS‐elicited RWPE‐1 cell injury model. Moreover, HDW administration impaired oxidative stress in the prostate and RWPE‐1 cells. In addition, HDW treatment activated the NRF2/ARE signaling in rat prostate tissue and cell models. Interestingly, NRF2/ARE pathway inhibitor ML385 reversed the inhibition effects of cell apoptosis, inflammation, and oxidative stress triggered by HDW. In summary, HDW alleviated inflammation and oxidative stress by activating NRF2/ARE signaling in CP/CPPS rat model and human prostate epithelial cell injury model.

中文翻译：

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白花蛇舌草通过 NRF2/ARE 信号通路抑制炎症和氧化应激，预防慢性前列腺炎

慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）是一种发病机制尚不清楚、症状反复发作的常见且严重的疾病。白花蛇舌草 (HDW) 因其在治疗各种慢性炎症性疾病方面的潜力而得到认可。本研究旨在探讨 HDW 治疗 CP/CPPS 的机制。采用弗氏完全佐剂(CFA)和LPS建立CP/CPPS大鼠和细胞模型。结果表明，HDW 降低了 CP 大鼠前列腺组织和 LPS 引发的 RWPE-1 细胞损伤模型中炎症相关因子的水平。此外，HDW 给药会损害前列腺和 RWPE-1 细胞的氧化应激。此外，HDW 治疗激活了大鼠前列腺组织和细胞模型中的 NRF2/ARE 信号传导。有趣的是，NRF2/ARE 通路抑制剂 ML385 逆转了 HDW 引发的细胞凋亡、炎症和氧化应激的抑制作用。总之，在CP/CPPS大鼠模型和人前列腺上皮细胞损伤模型中，HDW通过激活NRF2/ARE信号传导减轻炎症和氧化应激。