Amoxicillin, a member of the penicillin family, is primarily utilized for the treatment of various bacterial infections affecting ears, nose, throat, urinary tract, and skin. Given its widespread application in medicine, agriculture, environment, and food industry, the precise and sensitive detection of amoxicillin is important. This study introduces a novel approach to developing a sensitive and selective fluorescent aptasensor relying on fluorescence resonance energy transfer (FRET) for the specific detection of amoxicillin. The carboxyfluorescein-labeled aptamer serves as a energy donor, while MXene functions as an energy acceptor, and acting as a quencher. To achieve optimal detection efficiency, a dual optimization strategy utilizing RSM-CCD and ANN-GA was used to fine-tune experimental conditions. The fluorescence measurements revealed an expansive linear range extending from 100 to 2400 ng mL, accompanied by an exceptionally low detection limit of 1.53 ng mL. Additionally, it shows an excellent selectivity towards amoxicillin over other antibiotics commonly found in water matrices. The aptasensor demonstrates good stability and reproducibility; effectiveness of the aptasensor was validated by testing in real water samples. This remarkable sensitivity and broad dynamic range affirm the efficacy aptasensor in accurately detecting varying concentrations of amoxicillin in wastewater bodies.

中文翻译：

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基于 Ti3C2 MXene 的荧光适体传感器建模方法，用于水基质中的阿莫西林生物传感


阿莫西林是青霉素家族的一员，主要用于治疗影响耳、鼻、喉、尿道和皮肤的各种细菌感染。鉴于其在医药、农业、环境和食品工业中的广泛应用，阿莫西林的精确、灵敏检测具有重要意义。本研究介绍了一种新方法，开发依赖于荧光共振能量转移（FRET）的灵敏且选择性的荧光适体传感器，用于特异性检测阿莫西林。羧基荧光素标记的适体充当能量供体，而 MXene 充当能量受体，并充当猝灭剂。为了实现最佳检测效率，利用RSM-CCD和ANN-GA的双重优化策略来微调实验条件。荧光测量结果显示，线性范围从 100 到 2400 ng mL，检测限极低，为 1.53 ng mL。此外，与水基质中常见的其他抗生素相比，它对阿莫西林具有出色的选择性。适配体传感器具有良好的稳定性和重现性；通过在真实水样中进行测试，验证了适体传感器的有效性。这种卓越的灵敏度和广泛的动态范围证实了适体传感器在准确检测废水体中不同浓度的阿莫西林方面的功效。