The management of severe full-thickness skin defect wounds remains a challenge due to their irregular shape, uncontrollable bleeding, high risk of infection, and prolonged healing period. Herein, an all-in-one OD/GM/QCS@Exo hydrogel was prepared with catechol-modified oxidized hyaluronic acid (OD), methylacrylylated gelatin (GM), and quaternized chitosan (QCS) and loaded with adipose mesenchymal stem cell-derived exosomes (Exos). Cross-linking of the hydrogel was achieved using visible light instead of ultraviolet light irradiation, providing injectability and good biocompatibility. Notably, the incorporation of catechol groups and multicross-linked networks in the hydrogels conferred strong adhesion properties and mechanical strength against external forces such as tensile and compressive stress. Furthermore, our hydrogel exhibited antibacterial, anti-inflammatory, and antioxidant properties along with wound-healing promotion effects. Our results demonstrated that the hydrogel-mediated release of Exos significantly promotes cellular proliferation, migration, and angiogenesis, thereby accelerating skin structure reconstruction and functional recovery during the wound-healing process. Overall, the all-in-one OD/GM/QCS@Exo hydrogel provided a promising therapeutic strategy for the treatment of full-thickness skin defect wounds through actively participating in the entire process of wound healing.

中文翻译：

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负载外泌体的可见光可交联多功能水凝胶通过参与整个愈合过程促进全层皮肤缺损伤口愈合


由于其形状不规则、出血无法控制、感染风险高和愈合时间长，严重全层皮肤缺损伤口的处理仍然是一个挑战。在此，用儿茶酚修饰的氧化透明质酸（OD）、甲基丙烯酸明胶（GM）和季铵化壳聚糖（QCS）制备了一种一体式OD/GM/QCS@Exo水凝胶，并负载了脂肪间充质干细胞来源的水凝胶。外泌体（Exos）。使用可见光代替紫外线照射实现水凝胶的交联，提供可注射性和良好的生物相容性。值得注意的是，水凝胶中儿茶酚基团和多重交联网络的结合赋予了强大的粘附性能和抵抗拉应力和压应力等外力的机械强度。此外，我们的水凝胶还具有抗菌、抗炎和抗氧化特性以及促进伤口愈合的作用。我们的结果表明，水凝胶介导的 Exos 释放显着促进细胞增殖、迁移和血管生成，从而加速伤口愈合过程中的皮肤结构重建和功能恢复。总体而言，一体化 OD/GM/QCS@Exo 水凝胶通过积极参与伤口愈合的整个过程，为治疗全层皮肤缺损伤口提供了一种有前途的治疗策略。