We aimed at tabulating and characterizing a flavan-3-ol isolated from date seeds into date seed pills (DSP) as a commercial healthcare pill, and deeply studying their anti-glycoxidation impacts on bovine serum albumin using multi-dimensional approaches SDS-PAGE/NBT, UPLC-ESI-Q-TOF-MS, antiradical via Fenton-type reaction, multispectral, and molecular docking. Results showed that DSP were successfully tabulated (an encapsulation efficiency of 98.78 %) with high antioxidant values noncovalently binding with whey proteins supported by FTIR and lib-dock results. DSP dose-dependently decayed fluorescent-AGEs by 16.77, 5.32, and 37.41 % in DSP500-BSA-G/MGO/GO models, respectively, later confirmed by SDS-PAGE and initial precursors mitigation results. The docking results confirmed that DSP could bind with glycoxidation sites of BSA-subunits, namely Lys431, Arg458, and Arg196 H-bonds. DSP trapped both di-carbonyls and reduced CML-level, where new adducts such as epicatechin-mono/MGO-GO, were identified. 500 µg mL DSP reduced OH-induced fluorescence intensity of AGEs by 43.32 % in BSA-G/Fe/HO. Our results open a new direction for upcycling by-products as effective glycoxidation inhibitors in a type of healthcare pills in protein-rich models.

中文翻译：

---

Flavan-3-ol，一种来自枣子的黄烷醇，通过在 BSA-Fenton 液体模型中捕获其糖氧化位点、二羰基和 [rad]OH-自由基来脱糖化蛋白质


我们的目的是将从枣子中分离的黄烷-3-醇制成表格和表征为枣子丸（DSP）作为商业保健丸，并使用多维方法深入研究其对牛血清白蛋白的抗糖氧化影响 SDS-PAGE/ NBT、UPLC-ESI-Q-TOF-MS、通过芬顿型反应、多光谱和分子对接进行抗自由基。结果表明，DSP 成功制表（封装效率为 98.78%），具有高抗氧化值，与 FTIR 和 lib-dock 结果支持的乳清蛋白非共价结合。在 DSP500-BSA-G/MGO/GO 模型中，DSP 剂量依赖性荧光-AGE 衰减分别为 16.77、5.32 和 37.41%，随后通过 SDS-PAGE 和初始前体缓解结果得到证实。对接结果证实DSP可以与BSA亚基的糖氧化位点结合，即Lys431、Arg458和Arg196氢键。 DSP 捕获二羰基并降低 CML 水平，并鉴定出新的加合物，例如表儿茶素-单/MGO-GO。 500 µg mL DSP 将 BSA-G/Fe/H2O 中 OH 诱导的 AGE 荧光强度降低了 43.32%。我们的研究结果为在富含蛋白质的模型中将副产品升级为有效的糖氧化抑制剂开辟了新的方向，用于保健药丸。