The microbially induced calcium carbonate precipitation (MICP) technology is an emerging novel and sustainable technique for soil stabilization and remediation. MICP, a microorganism-mediated biomineralization process, has attracted interest for its potential to enhance soil characteristics. The inclusion of biochar, a carbon-rich substance formed by biomass pyrolysis, adds another degree of intricacy to this process. The study highlights the impact of the combination of biochar and MICP together, using a bacterium, , on soil improvement. This blend of MICP and biochar improved the soil in terms of its geotechnical properties and also enabled the sequestering of carbon safely. It was observed that addition of 4% biochar significantly increased the soil's shear strength parameters (c and φ) as well as its stiffness after 21 treatment cycles. This improvement was because the calcium carbonate precipitate, which acts as a crucial binding agent, increased significantly due to microbial action in the soil-biochar mixture compared to the pure soil sample. The excess carbonate precipitation on account of biochar addition was verified through SEM-EDAX analysis where the images showed noteworthy carbonate precipitation on the surface of particles and increment in the calcium mass at the same treatment cycles when compared with untreated sand. The collaboration between MICP and biochar effectively increased the carbon sequestration within the sand sample. It was observed that at 21 cycles of treatment, the carbon storage within the sand sample increased by almost 3 times at 4% biochar compared to sand without any biochar. The statistical analysis further affirmed that strength depends on both biochar and the number of treatment cycles, whereas carbon sequestration potential is primarily influenced by the biochar content alone. This strategy, as a sustainable and environmentally friendly approach, has the potential to reform soil improvement practices and contribute to both soil strength enhancement and climate change mitigation, supporting the maintenance of ecological balance.

中文翻译：

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生物炭对孢子八菌脲沉淀沙土方解石的影响


微生物诱导碳酸钙沉淀（MICP）技术是一种新兴的、可持续的土壤稳定和修复技术。 MICP 是一种微生物介导的生物矿化过程，因其增强土壤特性的潜力而引起了人们的兴趣。生物炭（一种由生物质热解形成的富含碳的物质）的加入，进一步增加了这一过程的复杂性。该研究强调了使用细菌将生物炭和 MICP 结合在一起对土壤改良的影响。 MICP 和生物炭的这种混合物改善了土壤的岩土特性，并能够安全地封存碳。据观察，在 21 个处理周期后，添加 4% 生物炭显着增加了土壤的剪切强度参数（c 和 φ）及其刚度。这种改善是因为与纯土壤样品相比，由于土壤-生物炭混合物中的微生物作用，作为关键粘合剂的碳酸钙沉淀物显着增加。通过 SEM-EDAX 分析验证了生物炭添加导致的过量碳酸盐沉淀，其中图像显示与未处理的砂相比，在相同处理周期下颗粒表面有明显的碳酸盐沉淀，并且钙质量增加。 MICP 和生物炭之间的合作有效地增加了沙样中的碳封存。据观察，在 21 个处理周期中，与不含任何生物炭的沙子相比，含有 4% 生物炭的沙子样品内的碳储存量增加了近 3 倍。 统计分析进一步证实，强度取决于生物炭和处理周期的数量，而碳封存潜力主要仅受生物炭含量的影响。该战略作为一种可持续且环境友好的方法，有可能改革土壤改良实践，有助于增强土壤强度和减缓气候变化，支持维持生态平衡。