Extracellular Vesicles (EV) have become an interesting focus as novel biomarkers of disease and are increasingly reported upon in humans and other species. The Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018) guidelines were published to improve rigor and standardisation within the EV field and provide a framework for the reliable isolation and characterisation of EV populations. However, this rigor and standardisation has been challenging in the area of comparative medicine. Herein we present the successful isolation of EVs from human and canine plasma using Size Exclusion Chromatography and characterise these EVs according to best international practice. This study provides evidence for the reliable comparison of human and canine EVs isolated by this approach, and a baseline description of the EVs from healthy dogs to inform future biomarker studies. This work also demonstrates that the MISEV2018 guidelines can be successfully applied to EVs isolated from canine plasma.

细胞外囊泡（EV）作为新型疾病生物标志物已成为人们关注的焦点，并且在人类和其他物种中的报道越来越多。 2018 年细胞外囊泡研究最小信息 (MISEV2018) 指南的发布是为了提高 EV 领域的严谨性和标准化，并为 EV 群体的可靠分离和表征提供框架。然而，这种严格性和标准化在比较医学领域一直面临挑战。在此，我们介绍了使用尺寸排阻色谱法从人和犬血浆中成功分离 EV 的方法，并根据最佳国际实践对这些 EV 进行了表征。这项研究为通过这种方法分离出的人类和犬类 EV 进行可靠比较提供了证据，并对健康狗的 EV 进行了基线描述，为未来的生物标志物研究提供信息。这项工作还表明，MISEV2018 指南可以成功应用于从犬血浆中分离出的 EV。