This work provides a sustainable method for producing iron oxide nanoparticles (FeONPs) using an extract from (AP) tubers. The nanoparticles were then thoroughly characterized by TGA/DSC, UV, FTIR, and SEM examination. They demonstrated their strong antibacterial properties against Gram-positive and Gram-negative microbes. The sizes of the ZOI were found to be 19, 25, 16 and 14 at 100 mg/mL as compared to cefoxitin as 18, 23, 8 and 12 mm for and respectively Their interaction with 2,2-diphenylpicrylhydrazyl (DPPH) revealed their free radical scavenging up to 70 %. NPs at 260 nm (λmax) have shown a hypo-chromic effect in the spectrophotometric approach, suggesting that FeONPs are intercalating in nature. The fluorescence quenching tests indicated the development of a non-fluorescent complex, implying FeONPs interaction with human serum albumin (HSA). As per Scatchard parameters, five binding sites observed in HSA during the interaction with FeONPs, where the K value indicated the NPs strong binding strength. The HSA and DPPH interaction nature of FeONPs suggests that they might be helpful in medical applications.

中文翻译：

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HSA、自由基以及与芍药合成的氧化亚铁纳米颗粒的抗菌相互作用


这项工作提供了一种利用 (AP) 块茎提取物生产氧化铁纳米颗粒 (FeONP) 的可持续方法。然后通过 TGA/DSC、UV、FTIR 和 SEM 检查对纳米颗粒进行彻底表征。他们展示了对革兰氏阳性和革兰氏阴性微生物的强大抗菌特性。 100 mg/mL 时，ZOI 的大小为 19、25、16 和 14，而头孢西丁的大小分别为 18、23、8 和 12 mm。它们与 2,2-二苯基三硝苯肼 (DPPH) 的相互作用揭示了它们的自由基清除率高达 70%。 260 nm (λmax) 的 NP 在分光光度法中显示出低色效应，表明 FeONP 本质上是嵌入的。荧光猝灭测试表明非荧光复合物的形成，这意味着 FeONPs 与人血清白蛋白 (HSA) 相互作用。根据 Scatchard 参数，在与 FeONP 相互作用期间，在 HSA 中观察到了五个结合位点，其中 K 值表明 NP 具有较强的结合强度。 FeONP 的 HSA 和 DPPH 相互作用性质表明它们可能有助于医学应用。