Cumulative light exposure, including electronic device radiation, may induce retinal pigment epithelium (RPE)-related retinal diseases, potentially mitigated by lutein. However, there is limited 3D RPE monolayer model to study the effect of phytochemicals on their activity and action mechanisms . Here, we present a method using a monolayer RPE tissue model on a 3D-printed polycaprolactone scaffold. Six-hour of phone screen light increased reactive oxygen species (ROS) in RPE cells, leading to oxidative stress, cytotoxicity, and inhibited metabolism. Pre-treating RPE cells (24 h) with 0.5 µM lutein reduced ROS levels and maintained viability under the same light exposure. Additionally, lutein demonstrated protective effects on crucial RPE functions, including phagocytosis and the retinal-blood barrier. This study underscores the importance of optimal lutein content in shielding RPE from phone screen light damage, and our model holds promise for evaluating the efficacy of antioxidants against light radiation.

中文翻译：

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叶黄素对手机屏幕光诱导的 3D 生物打印视网膜色素上皮单层损伤的保护作用


累积的光暴露，包括电子设备辐射，可能会诱发视网膜色素上皮 (RPE) 相关的视网膜疾病，叶黄素可能会减轻这种疾病。然而，用于研究植物化学物质对其活性和作用机制的影响的3D RPE单层模型有限。在这里，我们提出了一种在 3D 打印的聚己内酯支架上使用单层 RPE 组织模型的方法。六小时的手机屏幕光会增加 RPE 细胞中的活性氧 (ROS)，导致氧化应激、细胞毒性和新陈代谢受到抑制。用 0.5 µM 叶黄素预处理 RPE 细胞（24 小时）可降低 ROS 水平，并在相同的光照下保持活力。此外，叶黄素对关键的 RPE 功能（包括吞噬作用和视网膜血屏障）具有保护作用。这项研究强调了最佳叶黄素含量对于保护 RPE 免受手机屏幕光损伤的重要性，并且我们的模型有望评估抗氧化剂对抗光辐射的功效。