Ancient ethnobotanical practices handed down through traditional knowledge are still commonly employed to treat various pathologies, although the scientific reasons underlying their biological effects have not been clarified yet. In this contribution, the potential antitumoral activity of the aqueous extract from (AAE) was investigated to validate the hypothesis of the Algerian folk medicine which would suggest this plant derivative as a functional food for treating breast cancer. phytocomplex, isolated by maceration following exactly the African recipe, has been already characterized by our research group in previous works. Thus, the antiproliferative function of AAE against MDA-MB-231, a highly aggressive human breast adenocarcinoma cell line, was evaluated. Slowing down of cell growth, absence of cytotoxicity and DNA fragmentation, and cell cycle arrest at the G2/M phase were observed after treatment with AAE at different doses (0.3–6 mg of dried plant material equivalent per mL of culture medium) for 24 and 48 h. Wound and transwell assays proved that AAE possessed both antimigration and antiinvasive capacities, evidence also supported by molecular analyses focused on Metalloproteases (MMP-2 and MMP-9), Vimentin and ανβ3-Integrin. These results, together with the demonstration of the activation of p53/p21/p27 pathway and the increase of oxygen reactive species levels, suggested that AAE triggered a senescence process. The final confirmation was obtained by a specific kit staining senescent cells. All our data would explain the efficacy of the Algerian medicinal remedy based on the intake of the investigated functional plant food and would highlight the basics for developing novel natural pharmacological products based on AAE and showing preventive and therapeutic antineoplastic potentialities against highly aggressive breast cancers.

中文翻译：

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Anabasis articulata (Forssk.) 起订量食品水提取物触发氧化应激诱导的衰老并降低 MDA-MB-231 细胞的转移能力


通过传统知识传承下来的古代民族植物学实践仍然普遍用于治疗各种疾病，尽管其生物效应背后的科学原因尚未阐明。在这篇文章中，研究了 (AAE) 水提取物的潜在抗肿瘤活性，以验证阿尔及利亚民间医学的假设，该假设表明这种植物衍生物作为治疗乳腺癌的功能性食品。我们的研究小组在之前的工作中已经对植物复合物进行了表征，该植物复合物是按照非洲配方通过浸渍分离出来的。因此，评估了 AAE 针对高度侵袭性人乳腺癌细胞系 MDA-MB-231 的抗增殖功能。用不同剂量的 AAE（每毫升培养基 0.3–6 毫克干植物材料当量）处理 24 年后，观察到细胞生长减慢、没有细胞毒性和 DNA 断裂以及细胞周期停滞在 G2/M 期。和 48 小时。伤口和 Transwell 实验证明 AAE 具有抗迁移和抗侵袭能力，针对金属蛋白酶（MMP-2 和 MMP-9）、波形蛋白和 ανβ3-整合素的分子分析也支持了这一证据。这些结果与 p53/p21/p27 途径的激活和氧活性物质水平的增加一起表明 AAE 触发了衰老过程。最终的确认是通过特定的试剂盒对衰老细胞进行染色来获得的。 我们所有的数据都将根据所研究的功能性植物性食品的摄入量来解释阿尔及利亚药物的功效，并强调开发基于 AAE 的新型天然药物产品的基础知识，并显示出针对高度侵袭性乳腺癌的预防和治疗抗肿瘤潜力。