This study screened two probiotic preparations and comprehensively evaluated for anti-obesity efficacy in high-fat diet-induced obese mice. Obese mice were orally administered with low, medium, and high doses of AB-Kefir (ABK) or TWK10 (TWK), respectively. After 8 weeks of administration, medium doses and above of ABK and TWK significantly reduced body weight, white adipose tissue weight, serum glucose, and lipid levels and attenuated hepatic steatosis and adipocyte hypertrophy in obese mice without affecting appetite. ABK and TWK also decreased serum adipokine levels and increased the adiponectin/leptin ratio to ameliorate obesity-induced adipose tissue dysfunction and chronic inflammation. Moreover, ABK and TWK reduced adipogenesis/lipogenesis-related transcription factors and enzymes in adipose tissue and improved energy metabolism by activating the AMPK pathway. In particular, TWK showed superior anti-obesity activity compared to ABK. The outcomes of this study will help explore potential anti-obesity probiotic functional foods.

中文翻译：

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评估和比较两种益生菌制剂对高脂饮食诱导的肥胖小鼠的抗肥胖作用

本研究筛选了两种益生菌制剂，并综合评价其对高脂饮食诱导的肥胖小鼠的抗肥胖功效。肥胖小鼠分别口服低、中、高剂量的AB-Kefir (ABK) 或TWK10 (TWK)。给药8周后，中等及以上剂量的ABK和TWK显着降低肥胖小鼠的体重、白色脂肪组织重量、血糖和血脂水平，减轻肝脏脂肪变性和脂肪细胞肥大，且不影响食欲。 ABK 和 TWK 还可以降低血清脂肪因子水平并增加脂联素/瘦素比率，以改善肥胖引起的脂肪组织功能障碍和慢性炎症。此外，ABK 和 TWK 减少脂肪组织中的脂肪生成/脂肪生成相关转录因子和酶，并通过激活 AMPK 途径改善能量代谢。特别是，与 ABK 相比，TWK 显示出更优异的抗肥胖活性。这项研究的结果将有助于探索潜在的抗肥胖益生菌功能食品。