Maternal diet is indispensable for the proper development of offspring, and the establishment of a well-functioning intestinal barrier is crucial for normal growth and development. Edible bird’s nest (EBN) has positive impacts on intestinal health, but the underlying mechanisms are unknown. Our study showed that maternal EBN supplementation downregulated the serum levels of diamine oxidase and D-lactate, elevated expression of tight junction proteins ZO-1, Occludin, and Claudin-1, and regulated the intestinal microbiota in rat offspring. Spearman’s correlation analysis revealed that Claudin-1, transforming growth factor-β (TGF-β), and transcription factor RUNX3 were positively correlated with changes in intestinal bacteria. EBN may upregulate the expression of TGF-β by modulating the intestinal microbiota composition, enhancing RUNX3 binding to the Claudin-1 promoter region to upregulate its expression. In conclusion, we demonstrated that maternal EBN supplementation during gestation and lactation enhanced intestinal barrier function by upregulating Claudin-1 in rat offspring.

中文翻译：

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妊娠期和哺乳期母亲补充食用燕窝可通过上调大鼠后代的 Claudin-1 来增强肠道屏障功能


母体饮食对于后代的正常发育不可或缺，而建立功能良好的肠道屏障对于正常生长发育至关重要。食用燕窝（EBN）对肠道健康有积极影响，但其潜在机制尚不清楚。我们的研究表明，母体 EBN 补充剂下调了二胺氧化酶和 D-乳酸的血清水平，升高了紧密连接蛋白 ZO-1、Occludin 和 Claudin-1 的表达，并调节了大鼠后代的肠道微生物群。 Spearman的相关分析显示，Claudin-1、转化生长因子-β（TGF-β）和转录因子RUNX3与肠道细菌的变化呈正相关。 EBN 可能通过调节肠道微生物群组成、增强 RUNX3 与 Claudin-1 启动子区域的结合来上调 TGF-β 的表达，从而上调其表达。总之，我们证明，妊娠期和哺乳期母体 EBN 补充剂可通过上调大鼠后代的 Claudin-1 来增强肠道屏障功能。