Cocoa pod husk (CPH) and cocoa bean shell (CBS) are by-products obtained during pre-processing and processing of cocoa beans. Several bioactive compounds have been identified in these by-products that can be used for commercial applications as a way to promote the circular economy. Therefore, the objective of this paper was to recover bioactive compounds from CPH and CBS by sonoextraction process, to determine the type, content, and antioxidant activity in optimized extracts. To achieve our purpose, an optimization strategy using Box-Behnken Design coupled response surface methodology (MRS) was applied. The extraction conditions were optimized. The results obtained for CBS were: TPC (193 mg GAE/g), TEAC (1.02 mmol TE/g), FRAP (1.02 mmol FeSO4/g) and ORAC (2.6 mmol TE/g), while for CPH, the reported values were: TPC (48 mg GAE/g), TEAC (0.30 mmol TE/g), FRAP (0.35 mmol FeSO/g) and ORAC (0.43 mmol TE/g) under the optimized conditions: Time (X): 15 min, Amplitude (X): 80 %, Ethanol (X): 50 %. The LC-ESI-qTOF-MS analysis results allowed the identification of 79 compounds, of which 39 represent the CBS extract, while 40 compounds were identified in CPH extract. To conclude, sonotrode based extraction could be considered as an efficient and fast alternative for the recovery of bioactive substances from CBS and CPH.

中文翻译：

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通过超声波发生器优化提取和综合表征，从可可副产品中提取高潜力提取物


可可豆荚壳（CPH）和可可豆壳（CBS）是可可豆预处理和加工过程中获得的副产品。这些副产品中已鉴定出多种生物活性化合物，可用于商业应用，作为促进循环经济的一种方式。因此，本文的目的是通过超声提取工艺从CPH和CBS中回收生物活性化合物，以确定优化提取物中的类型、含量和抗氧化活性。为了实现我们的目的，我们采用了 Box-Behnken Design 耦合响应面方法 (MRS) 的优化策略。对提取条件进行了优化。 CBS 获得的结果为：TPC (193 mg GAE/g)、TEAC (1.02 mmol TE/g)、FRAP (1.02 mmol FeSO4/g) 和 ORAC (2.6 mmol TE/g)，而对于 CPH，报告值为：优化条件下的 TPC (48 mg GAE/g)、TEAC (0.30 mmol TE/g)、FRAP (0.35 mmol FeSO/g) 和 ORAC (0.43 mmol TE/g)：时间 (X)：15 分钟，振幅 (X)：80%，乙醇 (X)：50%。 LC-ESI-qTOF-MS 分析结果鉴定出 79 种化合物，其中 39 种代表 CBS 提取物，而在 CPH 提取物中鉴定出 40 种化合物。总之，基于超声波发生器的提取可被视为从 CBS 和 CPH 中回收生物活性物质的有效且快速的替代方案。