We report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction force into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral wave behavior of the cardiac wave.

中文翻译：

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通过单像素光学示踪剂 (SPOT) 实现大视场亚细胞牵引力摄像


我们报告了一个巨大的视场和高速摄像平台，用于以每秒 83 帧的速度测量超过 10,000 个超过 13 毫米的生物细胞的亚细胞牵引力。我们的单像素光学示踪剂 (SPOT) 工具使用嵌入软基板中的二维衍射光栅，将细胞的机械牵引力转换为摄像机可检测到的光学颜色。该平台测量不同细胞类型的亚细胞牵引力，包括紧密连接的组织片和接近孤立的细胞。我们使用该平台探索新生大鼠心室肌细胞（NRVM）紧密连接片中的机械波传播，发现某些组织区域的激活时间与心脏波的整体螺旋波行为不同。