, widely distributed in tropical and subtropical ecosystems, is capable of causing the fatal zoonotic disease melioidosis and exhibiting a global trend of dissemination. Rapid and sensitive detection of . is essential for environmental monitoring as well as infection control. Here, we developed an innovative biosensor for quantitatively detecting . relies on ATP released triggered by bacteriophage-induced bacteria lysis. The lytic bacteriophage vB_BpP_HN01, with high specificity, is employed alongside magnetic nanoparticles assembly to create a biological receptor, facilitating the capture and enrichment of viable target bacteria. Following a brief extraction and incubation process, the captured target undergoes rapid lysis to release contents including ATP. The EXPAR-CRISPR cascade reaction provides an efficient signal transduction and dual amplification module that allowing the generated ATP to guide the signal output as an activator, ultimately converting the target bacterial amount into a detectable fluorescence signal. The proposed bacteriophage affinity strategy exhibited superior performance for . detection with a dynamic range from 10^2 to 10^7 CFU mL, and a LOD of 45 CFU mL within 80 min. Moreover, with the output signal compatible across various monitoring methods, this work offers a robust assurance for rapid diagnosis and on-site environmental monitoring of . .

中文翻译：

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磁修饰噬菌体触发的 ATP 释放激活 EXPAR-CRISPR/Cas14a 系统，用于视觉检测类鼻疽伯克霍尔德氏菌

广泛分布于热带和亚热带生态系统中，能够引起致命的人畜共患类鼻疽病，并呈现全球传播趋势。快速、灵敏的检测。对于环境监测和感染控制至关重要。在这里，我们开发了一种用于定量检测的创新生物传感器。依赖于噬菌体诱导的细菌裂解触发的 ATP 释放。具有高特异性的裂解噬菌体 vB_BpP_HN01 与磁性纳米粒子组装一起用于创建生物受体，促进活目标细菌的捕获和富集。经过短暂的提取和孵育过程后，捕获的靶标会快速裂解，释放出包括 ATP 在内的内容物。 EXPAR-CRISPR级联反应提供了高效的信号转导和双重放大模块，使生成的ATP作为激活剂引导信号输出，最终将目标细菌量转化为可检测的荧光信号。所提出的噬菌体亲和策略表现出优越的性能。 80 分钟内检测动态范围为 10^2 至 10^7 CFU mL，LOD 为 45 CFU mL。此外，输出信号兼容多种监测方式，为快速诊断和现场环境监测提供了有力保障。 。