Environmental antibiotics and antibiotic resistance genes (ARGs) pose considerable threat to humans and animals; thus, the rapid and sensitive parallel detection of these pollutants from a single sample is urgently required. However, traditional multiplexed analytic technologies detect only one type of target (e.g., small molecules or nucleic acids) per assay. To address this issue, Evanescent wave Dual-color fluorescence Fiber-embedded Optofluidic Nanochip (EDFON) was fabricated by integrating a fiber-embedded optofluidic nanochip with evanescent wave dual-color fluorescence technology. The EDFON was used for the parallel quantitative detection of sulfamerazine (SMR) and MCR-1 with high sensitivity and specificity by combining a heterogeneous immunoassay with a homogenous hybridization chain reaction based on time-resolved effects. LODs of 0.032 μg/L and 35 pM was obtained for SMR and MCR-1, respectively, within 20 min. To our best knowledge, the EDFON is the first device for the simultaneous detection of two type of targets in each test, which is highly valuable to prevent the global threats of antibiotics and ARGs. Comparison with liquid chromatography-mass spectrometry showed a strong linear relationship (R = 0.998) for SMR pollution in the Qinghe River, with spiked SMR and MCR-1 negative surface and wastewater samples showing recovery rates of 91.8–113.4%. These results demonstrate the excellent accuracy and reliability of the EDFON, with features such as multi-analyte detection, field-deployment, and minimal-equipment, rendering it revolutionary for environmental monitoring, food safety, and medical diagnostics.

中文翻译：

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利用倏逝波双色荧光光纤嵌入光流控纳米芯片快速灵敏地并行现场检测水生环境中的抗生素和耐药基因

环境抗生素和抗生素抗性基因（ARG）对人类和动物构成相当大的威胁；因此，迫切需要从单个样品中快速、灵敏地平行检测这些污染物。然而，传统的多重分析技术每次测定仅检测一种类型的靶标（例如，小分子或核酸）。为了解决这个问题，倏逝波双色荧光光纤嵌入式光流控纳米芯片（EDFON）通过将光纤嵌入光流控纳米芯片与倏逝波双色荧光技术相结合而制成。 EDFON通过将异相免疫分析与基于时间分辨效应的均相杂交链式反应相结合，对磺胺嘧啶（SMR）和MCR-1进行平行定量检测，具有高灵敏度和特异性。在 20 分钟内，SMR 和 MCR-1 的 LOD 分别为 0.032 μg/L 和 35 pM。据我们所知，EDFON 是第一个在每次测试中同时检测两种类型目标的设备，这对于防止抗生素和 ARG 的全球威胁非常有价值。与液相色谱-质谱法的比较显示，清河中 SMR 污染存在很强的线性关系（R = 0.998），加标的 SMR 和 MCR-1 阴性地表和废水样品的回收率为 91.8-113.4%。这些结果证明了 EDFON 卓越的准确性和可靠性，以及多分析物检测、现场部署和最少设备等功能，使其在环境监测、食品安全和医疗诊断方面具有革命性意义。