is one of the primary pathogens causing postharvest blue mold of pears, which leads to the spoiling of pulp and produces patulin, endangering food security. Mitogen-activated protein kinase (MAPK) signaling pathway regulates the growth and development, cell wall integrity, and pathogenicity of fungi. Mitogen-activated protein kinase kinase (MKK) in the MAPK signaling pathway has been reflected to be associated with fungal cell wall integrity. Nevertheless, the pivotal function of in the process of infection remained unexplored. In this study, was knocked out and repaired by the -mediated homologous recombination technology to explore the function of in infection process. It has been found that the deletion of induces morphological changes in the mutant strain and delayed mycelium and spore growth. Further studies showed that the mutant strain was susceptible to cell wall interference compounds and showed a significant decrease in decay diameters and virulence during the infection of the pears. RT-qPCR analysis showed that the deletion of affected the relative expression levels of its upstream and downstream genes. Our results suggest that is essential in the growth and development, cell wall integrity, pathogenicity, and toxigenic capacity of .

中文翻译：

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MAP 激酶 MKK：感染梨的扩展青霉的发育、产毒潜力和发病机制的中心调节因子

是引起梨采后蓝霉病的主要病原菌之一，导致果肉腐败并产生棒曲霉素，危害粮食安全。丝裂原激活蛋白激酶（MAPK）信号通路调节真菌的生长发育、细胞壁完整性和致病性。 MAPK 信号通路中的丝裂原激活蛋白激酶激酶 (MKK) 已被证明与真菌细胞壁完整性相关。然而，其在感染过程中的关键功能仍有待探索。本研究通过介导的同源重组技术对其进行敲除和修复，探讨其在感染过程中的功能。已经发现，缺失会引起突变菌株的形态变化并延迟菌丝体和孢子的生长。进一步的研究表明，突变菌株对细胞壁干扰化合物敏感，并且在感染梨期间腐烂直径和毒力显着降低。 RT-qPCR分析表明，缺失影响了其上下游基因的相对表达水平。我们的结果表明，它对于 的生长和发育、细胞壁完整性、致病性和产毒能力至关重要。