The increasing food allergic cases have posed a great challenge to the human health worldwide. Avoiding the exposure to food allergens is still the only efficient method to prevent food allergic reactions for food allergic sufferers because there are no effective treatments for food allergy up to now. Therefore, it is crucial to develop analytical technologies for rapid and on-site detection of food allergens, helping allergy-prone groups make proper option when buying or eating foods. Recently, various detection technologies and portable analytical devices have been widely developed for rapid, accurate, and on-site screening of food allergens. In this review, first of all, the traditional detection methods were simply introduced and discussed. Then, the latest advances on emerging biosensing technologies (i.e., colorimetric biosensing, fluorescent biosensing, electrochemical biosensing, surface enhanced Raman spectroscopy (SERS) biosensing, surface plasmon resonance (SPR) biosensing, and dual-mode biosensing) and portable analytical devices (such as lateral flow assays (LFAs), microfluidic chips, and paper-based microfluidic devices) for the detection of food allergens were comprehensively summarized. Furthermore, the advantages and weaknesses of these analytical methods for food allergen detection were also compared and discussed. Most importantly, the remaining challenges and chances in this important field were also proposed. Emerging biosensing technologies have shown a great potential in the on-site detection of food allergens. Impressively, the integration of biosensing technologies and portable detection devices (e.g., LFAs, microfluidic chips, and paper-based microfluidic devices) is one of the most promising methods for reliable and on-site detection of food allergens.

中文翻译：

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用于检测食物过敏原的新兴生物传感技术和便携式分析设备的最新进展


食物过敏病例的不断增加给全球人类健康带来了巨大挑战。对于食物过敏患者来说，避免接触食物过敏原仍然是预防食物过敏反应的唯一有效方法，因为目前还没有有效的治疗食物过敏的方法。因此，开发快速现场检测食物过敏原的分析技术至关重要，帮助易过敏人群在购买或食用食物时做出正确选择。近年来，各种检测技术和便携式分析设备得到广泛发展，用于快速、准确、现场筛查食物过敏原。本文首先对传统的检测方法进行了简单的介绍和讨论。然后，新兴生物传感技术（即比色生物传感、荧光生物传感、电化学生物传感、表面增强拉曼光谱（SERS）生物传感、表面等离子共振（SPR）生物传感和双模式生物传感）和便携式分析设备（例如全面总结了用于检测食物过敏原的横向流动测定（LFA）、微流控芯片和纸基微流控装置）。此外，还比较和讨论了这些食品过敏原检测分析方法的优缺点。最重要的是，还提出了这一重要领域中剩余的挑战和机遇。新兴的生物传感技术在食品过敏原的现场检测方面显示出巨大的潜力。令人印象深刻的是，生物传感技术和便携式检测设备（例如，、LFA、微流控芯片和纸基微流控装置）是可靠地现场检测食物过敏原的最有前途的方法之一。