Food safety is a global health issue. The major causes of foodborne diseases, including bacteria, viruses, parasites, prions, and chemicals in unsafe food, lead to severe outbreaks worldwide annually. Traditional detection technologies such as polymerase chain reaction (PCR) rely on complex thermal apparatus, hindering their applications in novel integrated devices and high-throughput analysis for point-of-care tests for foodborne pathogenic bacteria and viruses. Isothermal nucleic acid amplification-based lab-on-a-chip (LOC) technology represents an alternative approach to on-site detection, as it does not require programmed temperature control. In addition, miniaturized microfluidic LOC can reduce the use of reagents and the need of other expensive equipment. We summarized the recent progress in the application of isothermal nucleic acid amplification-based microfluidic LOC devices used in agri-foods for the detection of pathogenic bacteria and viruses. The potential and limitations of these methods were also analyzed. Nucleic acid sequence-based amplification (NASBA), hybridization chain reaction (HCR), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), and loop-mediated isothermal amplification (LAMP)-based LOC devices have been successfully developed and applied for the detection of pathogenic bacteria and viruses in agri-foods due to their high sensitivity, specificity, and rapid response. By integrating sample pre-processing and extraction either before or on a single chip, it becomes possible to minimize interference signals from food sample matrix before the nucleic acid amplification step. Further optimization and development hold the potential to improve the performance of these devices, expanding their uses in the surveillance and control of foodborne or food-related diseases.

中文翻译：

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基于等温核酸扩增的微流控“芯片实验室”，用于检测农产品中的致病细菌和病毒

食品安全是一个全球性的健康问题。食源性疾病的主要原因，包括细菌、病毒、寄生虫、朊病毒和不安全食品中的化学物质，每年都会在世界范围内导致严重的疫情爆发。聚合酶链反应（PCR）等传统检测技术依赖于复杂的热设备，阻碍了其在新型集成设备和食源性致病菌和病毒即时检测高通量分析中的应用。基于等温核酸扩增的芯片实验室 ​​(LOC) 技术代表了现场检测的另一种方法，因为它不需要程序温度控制。此外，小型化微流控LOC可以减少试剂的使用和其他昂贵设备的需求。我们总结了基于等温核酸扩增的微流控LOC装置在农产品中用于病原菌和病毒检测的应用的最新进展。还分析了这些方法的潜力和局限性。基于核酸序列扩增（NASBA）、杂交链式反应（HCR）、滚环扩增（RCA）、重组酶聚合酶扩增（RPA）、环介导等温扩增（LAMP）的LOC装置已成功开发并应用由于其高灵敏度、特异性和快速响应，用于检测农产品中的致病细菌和病毒。通过在单个芯片之前或在单个芯片上集成样​​品预处理和提取，可以在核酸扩增步骤之前最大限度地减少来自食品样品基质的干扰信号。进一步的优化和开发有可能提高这些设备的性能，扩大其在食源性或食品相关疾病的监测和控制中的用途。