In this study, the impact of three unsaturated fatty acids (Oleic acid: OA, Eicosapentaenoic acid: EPA, Docosahexaenoic acid: DHA) on the oxidation and structure of rainbow trout myofibrillar protein (MP) was explored. The findings revealed a notable increase in carbonyl content ( < 0.05) and a significant decrease in total sulfhydryl content (P < 0.05) of MP with the concentration increase of the three unsaturated fatty acids. Endogenous fluorescence spectroscopy and surface hydrophobicity analyses showed that unsaturated fatty acids can cause unfolding and exposure of hydrophobic groups in MP. In addition, SDS-PAGE showed that disulfide bonds were associated with MP cross-linking and aggregate size induced by unsaturated fatty acids. Overall, three unsaturated fatty acid treatments facilitated the oxidation of myofibrillar proteins, and the extent of protein oxidation was closely associated with the concentration of unsaturated fatty acids.

中文翻译：

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三种不饱和脂肪酸对虹鳟鱼（Oncorhynchus mykiss）蛋白质氧化和肌原纤维蛋白结构的影响

在本研究中，探讨了三种不饱和脂肪酸（油酸：OA、二十碳五烯酸：EPA、二十二碳六烯酸：DHA）对虹鳟鱼肌原纤维蛋白（MP）氧化和结构的影响。结果表明，随着三种不饱和脂肪酸浓度的增加，MP的羰基含量显着增加（<0.05），而总巯基含量显着降低（P<0.05）。内源荧光光谱和表面疏水性分析表明，不饱和脂肪酸可以引起MP中疏水基团的去折叠和暴露。此外，SDS-PAGE 显示二硫键与不饱和脂肪酸诱导的 MP 交联和聚集体大小相关。总体而言，三种不饱和脂肪酸处理促进了肌原纤维蛋白的氧化，并且蛋白质氧化的程度与不饱和脂肪酸的浓度密切相关。