Despite the worldwide occurrence and high genetic diversity of Bartonella spp. in bats, few studies investigate their occurrence in bat-associated mites. To date, 26 species of Macronyssidae mite species have been reported from Brazil, and 15 of which were found parasitizing bats. The present study aimed to investigate the presence of Bartonella DNA in bat-associated macronyssid mites from Brazil. For this purpose, 393 macronyssid specimens were selected by convenience from the tissue bank of the Acari Collection of the Instituto Butantan (IBSP). These mites were collected from 14 different bat species in three different Brazilian States (Minas Gerais, Paraná, and Rio de Janeiro). Out of 165 mites positive in the PCR for the endogenous 18S rRNA gene, only eight were positive in the qPCR for Bartonella spp. based on the nuoG gene, and we were able to obtain two sequences base in this same gene, and one sequence based on the 16S rRNA gene. The phylogenetic inference based on the nuoG gene grouped the obtained sequences with Bartonella genotypes previously detected in bats and associated bat flies, while the phylogeny based on the 16S rRNA grouped the obtained sequence in the same clade of Bartonella genotypes previously detected in Dermanyssus gallinae. These findings suggest that macronyssid mites might be associated with the maintenance of bartonellae among bats.

尽管巴尔通体在世界范围内存在且遗传多样性很高。在蝙蝠中，很少有研究调查它们在与蝙蝠相关的螨虫中的发生情况。迄今为止，巴西已报告26种Macromyssidae螨类物种，其中15种被发现寄生于蝙蝠。本研究旨在调查来自巴西的与蝙蝠相关的巨眼螨中巴尔通体DNA的存在。为此，我们从布坦坦研究所 (IBSP) 螨虫保藏中心的组织库中方便地挑选了 393 个大袋螨标本。这些螨虫是从巴西三个不同州（米纳斯吉拉斯州、巴拉那州和里约热内卢）的 14 种不同蝙蝠物种中采集的。 165 只螨虫的内源 18S rRNA 基因 PCR 呈阳性，其中只有 8 只螨虫的巴尔通体 qPCR 呈阳性。基于nuoG基因，我们能够获得基于该同一基因的两个序列，以及基于16S rRNA基因的一个序列。基于nuoG基因的系统发育推断将获得的序列与先前在蝙蝠和相关蝙蝠蝇中检测到的巴尔通体基因型分组，而基于16S rRNA的系统发育推断将获得的序列分组在先前在鸡皮皮螨中检测到的巴尔通体基因型的同一进化支中。这些发现表明，大袋螨可能与蝙蝠巴尔通体的维持有关。