Hypothyroidism is the most commonly diagnosed endocrine disease in dogs. The objective of this study was to evaluate the changes in the redox status in canine hypothyroidism using whole blood (WB) and red blood cell (RBCs) lysates. For this purpose, a panel of five antioxidants and five oxidants biomarkers was measured in WB and RBCs lysates of 30 dogs with hypothyroidism, 26 dogs with non-thyroidal illnesses and 15 healthy dogs. The antioxidants measured were cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type-1 (PON-1). Oxidants measured include the total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived metabolites (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). WB showed a significant decrease of the antioxidants CUPRAC, TEAC and thiol, and also an increase in TBARS and a decrease in AOPP in dogs with hypothyroidism compared to healthy dogs. Meanwhile, RBCs lysates showed a significant increase in FRAP and PON-1 in dogs with hypothyroidism. The changes in the redox biomarkers in this study show that WB in canine hypothyroidism had a higher number of changes in biomarkers of the redox status than RBCs lysates, making it a promising sample type for the evaluation of the redox status in this disease. In addition, WB is easier and simpler to process than RBCs lysates and unlike serum, it does not have any hemolysis interference.

甲状腺功能减退症是犬最常见的内分泌疾病。本研究的目的是使用全血（WB）和红细胞（RBC）裂解物评估犬甲状腺功能减退症氧化还原状态的变化。为此，我们测量了 30 只患有甲状腺功能减退症的狗、26 只患有非甲状腺疾病的狗和 15 只健康狗的 WB 和 RBC 裂解物中的一组五种抗氧化剂和五种氧化剂生物标志物。测量的抗氧化剂是铜还原抗氧化能力（CUPRAC）、血浆铁还原能力（FRAP）、Trolox当量抗氧化能力（TEAC）、硫醇和1型对氧磷酶（PON-1）。测量的氧化剂包括总氧化剂状态（TOS）、过氧化物活性（POX-Act）、活性氧衍生代谢物（d-ROM）、高级氧化蛋白产物（AOPP）和硫代巴比妥酸反应物质（TBARS）。 WB 显示，与健康狗相比，甲状腺功能减退症狗的抗氧化剂 CUPRAC、TEAC 和硫醇显着减少，TBARS 增加，AOPP 减少。同时，甲状腺功能减退犬的红细胞裂解物中 FRAP 和 PON-1 显着增加。本研究中氧化还原生物标志物的变化表明，犬甲状腺功能减退症中的WB比红细胞裂解物具有更多数量的氧化还原状态生物标志物变化，使其成为评估该疾病氧化还原状态的有前景的样本类型。此外，WB 比红细胞裂解物更容易、更简单地处理，并且与血清不同，它没有任何溶血干扰。