SUMMARYThe Q transcription factor plays important roles in improving multiple wheat domestication traits such as spike architecture, threshability and rachis fragility. However, whether and how it regulates abiotic stress adaptation remain unclear. We found that the transcriptional expression of Q can be induced by NaCl and abscisic acid treatments. Using the q mutants generated by CRISPR/Cas9 and Q overexpression transgenic lines, we showed that the domesticated Q gene causes a penalty in wheat salt tolerance. Then, we demonstrated that Q directly represses the transcription of TaSOS1‐3B and reactive oxygen species (ROS) scavenging genes to regulate Na+ and ROS homeostasis in wheat. Furthermore, we showed that wheat salt tolerance protein TaWD40 interacts with Q to competitively interfere with the interaction between Q and the transcriptional co‐repressor TaTPL. Taken together, our findings reveal that Q directly represses the expression of TaSOS1 and some ROS scavenging genes, thus causing a harmful effect on wheat salt tolerance.

中文翻译：

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Q通过直接抑制TaSOS1和活性氧清除基因的表达负向调节小麦耐盐性

摘要 Q 转录因子在改善多种小麦驯化性状（例如穗结构、脱粒性和穗轴脆性）方面发挥着重要作用。然而，它是否以及如何调节非生物胁迫适应仍不清楚。我们发现转录表达问可以通过 NaCl 和脱落酸处理诱导。使用qCRISPR/Cas9 产生的突变体和问过度表达转基因品系，我们表明驯化的问基因导致小麦耐盐性下降。然后，我们证明Q直接抑制转录TaSOS1-3B和活性氧 (ROS) 清除基因来调节 Na+和小麦中的ROS稳态。此外，我们发现小麦耐盐蛋白 TaWD40 与 Q 相互作用，竞争性干扰 Q 与转录共阻遏物 TaTPL 之间的相互作用。综上所述，我们的研究结果表明 Q 直接抑制塔SOS1以及一些ROS清除基因，从而对小麦的耐盐性造成有害影响。