Cell culture meat is based on the scaled-up expansion of seed cells. The biological differences between seed cells from large yellow croakers in the two-dimensional (2D) and three-dimensional (3D) culture systems have not been explored. Here, satellite cells (SCs) from large yellow croakers () were grown on cell climbing slices, hydrogels, and microcarriers for five days to analyze the biological differences of SCs on different cell scaffolds. The results exhibited that SCs had different cell morphologies in 2D and 3D cultures. Cell adhesion receptors (and) and adhesion spot markerof the 3D cultures were markedly expressed. Furthermore, myogenic decision markers (and) were significantly enhanced. However, the expression of myogenic differentiation marker () was significantly increased in the microcarrier group. Combined with the transcriptome data, this suggests that cell adhesion of SCs in 3D culture was related to the integrin signaling pathway. In contrast, the slight spontaneous differentiation of SCs on microcarriers was associated with rapid cell proliferation. This study is the first to report the biological differences between SCs in 2D and 3D cultures, providing new perspectives for the rapid expansion of cell culture meat-seeded cells and the development of customized scaffolds.

中文翻译：

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用于快速扩增大黄鱼卫星细胞的水凝胶/微载体细胞支架：2D 和 3D 细胞培养之间的差异分析

细胞培养肉基于种子细胞的放大扩增。二维（2D）和三维（3D）培养系统中大黄鱼种子细胞之间的生物学差异尚未被探索。在这里，来自大黄鱼的卫星细胞（SC）在细胞攀爬片、水凝胶和微载体上生长五天，以分析不同细胞支架上SC的生物学差异。结果表明，SCs 在 2D 和 3D 培养物中具有不同的细胞形态。 3D培养物的细胞粘附受体（和）和粘附点标记物显着表达。此外，肌源性决策标记（和）显着增强。然而，微载体组中生肌分化标记物()的表达显着增加。结合转录组数据，这表明 3D 培养中 SC 的细胞粘附与整合素信号通路有关。相反，微载体上 SC 的轻微自发分化与细胞快速增殖相关。该研究首次报道了2D和3D培养中SC之间的生物学差异，为细胞培养肉籽细胞的快速扩增和定制支架的开发提供了新的视角。