The impact of cold plasma (CP) treatment on anthocyanin accumulation in postharvest blueberries is well-documented. However, the underlying regulatory mechanisms are still not fully understood. To gain further insights, we conducted biochemistry, transcriptomic, and metabolomic analyses. The comparison between control and CP-treated samples revealed 948 differentially expressed genes (DEGs) and 20 differentially accumulated metabolites (DAMs) (Malvidin-3-O-galactoside, Cyanidin-3-O-galactoside, proanthocyanidin B1, peonidin-3-O-galactoside, etc.). A total of 63 DEGs may be involved in anthocyanins biosynthesis, including PAL, C4H, 4CL, CHS, CHI, F3H, F3’H, F3’5’H, FLS, DFR, LDOX, F3GT, UGT, and GST. The co-expression network analysis revealed that UGT plays an important role in the biosynthesis of anthocyanins in CP-treated blueberry at postharvest. These findings indicate that CP treatment promotes the accumulation of anthocyanins by affecting the phenylpropanoid pathways and flavonoid biosynthesis. This research provides fundamental insights into the molecular mechanisms underlying anthocyanin accumulation in blueberries following CP treatment.

中文翻译：

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介质阻挡放电冷等离子体对蓝莓花青素代谢的影响：有针对性的代谢组学和转录组学分析

冷等离子体（CP）处理对采后蓝莓花青素积累的影响已有充分记录。然而，潜在的监管机制仍不完全清楚。为了获得进一步的见解，我们进行了生物化学、转录组学和代谢组学分析。对照和 CP 处理样品之间的比较揭示了 948 个差异表达基因 (DEG) 和 20 个差异累积代谢物 (DAM)（Malvidin-3-O-半乳糖苷、Cyanidin-3-O-半乳糖苷、原花青素 B1、peonidin-3-O） -半乳糖苷等）。共有63个DEG可能参与花青素生物合成，包括PAL、C4H、4CL、CHS、CHI、F3H、F3'H、F3'5'H、FLS、DFR、LDOX、F3GT、UGT和GST。共表达网络分析表明，UGT 在 CP 处理的蓝莓采后花青素的生物合成中发挥着重要作用。这些发现表明，CP 处理通过影响苯丙素途径和类黄酮生物合成来促进花青素的积累。这项研究为 CP 处理后蓝莓中花青素积累的分子机制提供了基本见解。