Previously, we have shown that four phenylalanine aminolyases () in citrus fruit were involved in resistance to green mold through increased phenolics. Further, in transcriptome data, many genes expressed in the sugar metabolism and shikimate pathways were found to be significantly up-regulated in the overexpression groups. Therefore, this study explored the changes in these pathways during the process of overexpression of triggering disease resistance in fruit. It was found that the expression of important genes and enzyme activities in the sugar metabolism and shikimate pathways were up-regulated, various sugar contents were changed, and most amino acid contents were increased during the process. In conclusion, this study demonstrated that during the transient overexpression of on citrus to improve fruit resistance to green mold, the sucrose catabolic synthesis process, glucose catabolic utilization process, and shikimate pathway were activated, various sugar contents were significantly changed, and amino acid contents were increased.

中文翻译：

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采后柑橘果实中 CsPAL 基因瞬时过表达影响葡萄糖代谢和莽草酸途径的变化

此前，我们已经证明柑橘类水果中的四种苯丙氨酸氨基裂解酶通过增加酚类物质参与对绿霉的抵抗力。此外，在转录组数据中，发现在糖代谢和莽草酸途径中表达的许多基因在过表达组中显着上调。因此，本研究探讨了果实过度表达引发抗病性过程中这些途径的变化。结果发现，糖代谢和莽草酸途径中重要基因和酶活性的表达上调，各种糖含量发生变化，并且在此过程中大多数氨基酸含量增加。总之，本研究表明，在柑橘短暂过表达on以提高果实对绿霉的抗性的过程中，蔗糖分解代谢合成过程、葡萄糖分解代谢利用过程和莽草酸途径被激活，各种糖含量发生显着变化，氨基酸含量也发生变化。增加了。