can enhance the metabolism of organophosphate pesticides in plants, but the mechanism is unclear. Here, we performed high-throughput transcriptome sequencing of roots upon (TM) inoculation and phoxim (P) application in tomato ( L.). A total of 4059 differentially expressed genes (DEGs) were obtained, including 2110 up-regulated and 1949 down-regulated DEGs in P vs TM+P. COG and KOG analysis indicated that DEGs were mainly enriched in signal transduction mechanisms. We then focused on the pesticide detoxification pathway and screened out cytochrome P450 as a putative gene for functional analysis. We suppressed the expression of in tomato plants by virus-induced gene silencing and analyzed tissue-specific phoxim residues, oxidative stress markers, glutathione pool, GST activity and related gene expression. Silencing significantly increased phoxim residue and induced oxidative stress in tomato plants, by attenuating the TM-induced increased activity of antioxidant and detoxification enzymes, redox homeostasis and transcripts of detoxification genes including , , , , , , , , and . The study revealed a critical mechanism by which TM promotes the metabolism of phoxim in tomato roots, which can be useful for further understanding the -induced xenobiotic detoxification and improving food safety.

中文翻译：

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细胞色素 P450 CYP736A12 对于木霉诱导的辛硫磷植物毒性的减轻和番茄根部农药残留的减少至关重要

可以增强植物体内有机磷农药的代谢，但其作用机制尚不清楚。在这里，我们对番茄（L.）接种（TM）和辛硫磷（P）应用后的根进行高通量转录组测序。共获得4059个差异表达基因（DEG），其中P与TM+P相比上调2110个DEG，下调1949个DEG。 COG和KOG分析表明DEGs主要富集于信号转导机制。然后我们关注农药解毒途径并筛选出细胞色素P450作为功能分析的推定基因。我们通过病毒诱导的基因沉默抑制了番茄植株中的表达，并分析了组织特异性辛硫磷残基、氧化应激标记物、谷胱甘肽库、GST 活性和相关基因表达。沉默显着增加了辛硫磷残留并诱导了番茄植株中的氧化应激，通过减弱TM诱导的抗氧化和解毒酶、氧化还原稳态和解毒基因转录的活性增加，包括、、、、、、和。该研究揭示了TM促进番茄根部辛硫磷代谢的关键机制，有助于进一步了解TM诱导的异生物质解毒和提高食品安全。