Glioma is a common type of malignant tumor with high global incidence and limited therapeutic options. Scorpion is listed as medicinal and functional food material for centuries to relieve neurological diseases including glioma in traditional Chinese medicine. In this study, network pharmacology firstly revealed that Scorpion may exert anti-tumor effect via regulating oncogenic signals like EGFR and SRC. The anti-neoplastic effect and safety of Scorpion were further validated on two in vivo glioma models, subcutaneous xenograft and orthotopic transplanted glioma in C57BL/6J mice. Data from mRNA and miRNA transcriptomes of orthotopic glioma showed that exerted anti-glioma as well as immune regulatory effects. Flow cytometry and immunofluorescence analysis demonstrated that improved CD4 + and CD8 + T immunity and decreased myeloid-derived suppressor cells (MDSCs) infiltration in glioma. Collectively, Scorpion restrained the progress of glioma possibly via improving T cell surveillance and inhibiting MDSC in tumor microenvironment.

中文翻译：

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马氏虾 (BmK) 蝎子通过抑制肿瘤微环境中的骨髓源性抑制细胞和激活 T 细胞来发挥抗神经胶质瘤作用


胶质瘤是一种常见的恶性肿瘤，全球发病率较高，但治疗选择有限。数百年来，全蝎在中医中被列为治疗包括神经胶质瘤在内的神经系统疾病的药用和功能性食品原料。本研究通过网络药理学首次揭示Scorpion可能通过调节EGFR、SRC等致癌信号发挥抗肿瘤作用。 Scorpion 的抗肿瘤作用和安全性在两种体内胶质瘤模型（皮下异种移植和 C57BL/6J 小鼠原位移植胶质瘤）上得到进一步验证。原位神经胶质瘤的 mRNA 和 miRNA 转录组数据表明，其具有抗神经胶质瘤和免疫调节作用。流式细胞术和免疫荧光分析表明，神经胶质瘤中 CD4 + 和 CD8 + T 免疫力得到改善，骨髓源性抑制细胞 (MDSC) 浸润减少。总的来说，Scorpion 可能通过改善 T 细胞监测和抑制肿瘤微环境中的 MDSC 来抑制神经胶质瘤的进展。