Eggshell is one of the most important indicators of egg quality, and due to low shell strength, pimple eggs () are more susceptible to breakage, thus causing huge economic losses to the egg industry. At the current time, the molecular mechanisms that regulate the formation of pimple eggs are poorly understood. In this study, uterine tissues of PE-laying hens (n = 8) and normal egg () -laying hens (n = 8) were analyzed by whole transcriptome sequencing, and a total of 619 differentially expressed mRNAs (), 122 differentially expressed lncRNAs () and 21 differentially expressed miRNAs () were obtained. Based on the targeting relationship among DE mRNAs, DE lncRNAs and DE miRNAs, we constructed a competitive endogenous RNA () network including 12 DE miRNAs, 19 DE lncRNAs, and 128 DE mRNAs. Considering the large amount of information contained in the network, we constructed a smaller ceRNA network to better understand the complex mechanisms of pimple egg formation. The smaller ceRNA network network contains 7 DE lncRNAs (), 5 DE miRNAs () and 7 DE mRNAs (), and these differentially expressed genes may play key regulatory roles in the formation of pimpled eggs in hens. This study provides the overall expression profiles of mRNAs, lncRNAs and miRNAs in the uterine tissues of hens, which provides a theoretical basis for further research on the molecular mechanisms of pimpled egg formation, and has potential applications in improving eggshell quality.

中文翻译：

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全转录组测序揭示了与母鸡斑点蛋相关的关键基因和 ceRNA 调控网络

蛋壳是鸡蛋品质最重要的指标之一，而由于蛋壳强度低，疙瘩蛋()更容易破裂，从而给蛋业造成巨大的经济损失。目前，人们对调节丘疹卵形成的分子机制知之甚少。本研究对PE蛋鸡（n = 8）和正常蛋（n = 8）蛋鸡（n = 8）的子宫组织进行全转录组测序分析，共获得619个差异表达mRNA（），122个差异表达mRNA。获得了lncRNA()和21个差异表达的miRNA()。基于DE mRNA、DE lncRNA和DE miRNA之间的靶向关系，我们构建了一个包含12个DE miRNA、19个DE lncRNA和128个DE mRNA的竞争性内源RNA（）网络。考虑到网络中包含的大量信息，我们构建了一个更小的ceRNA网络，以更好地理解丘疹卵形成的复杂机制。较小的ceRNA网络包含7个DE lncRNA()、5个DE miRNA()和7个DE mRNA()，这些差异表达基因可能在母鸡疙瘩蛋的形成中发挥关键的调控作用。本研究提供了母鸡子宫组织中mRNA、lncRNA和miRNA的整体表达谱，为进一步研究疙瘩蛋形成的分子机制提供了理论基础，在改善蛋壳质量方面具有潜在的应用前景。