Insect resistance evolution poses a significant threat to the advantages of biopesticides and transgenic crops utilizing insecticidal Cry-toxins from (Bt). However, there is limited research on the relationship between transcriptional regulation of specific toxin receptors in lepidopteran insects and their resistance to Bt toxins. Here, we report the positive regulatory role of the SfGATAe transcription factor on the expression of the ABCC2 gene in . DNA regions in the promoter that are vital for regulation by SfGATAe, utilizing DAP-seq technology and promoter deletion mapping. Through yeast one-hybrid assays, DNA pull-down experiments, and site-directed mutagenesis, we confirmed that the transcription factor SfGATAe regulates the core control site PBS2 in the target gene. Tissue-specific expression analysis has revealed that SfGATAe is involved in the regulation and expression of midgut cells in the fall armyworm. Silencing in fall armyworm larvae resulted in reduced expression of and decreased sensitivity to Cry1Ac toxin. Overall, this study elucidated the regulatory mechanism of the transcription factor SfGATAe on the expression of the toxin receptor gene and this transcriptional control mechanism impacts the resistance of the fall armyworm to Bt toxins.

中文翻译：

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转录因子 SfGATAe 通过减少 ABCC2 表达对草地贪夜蛾 Bt Cry 毒素抗性的贡献

昆虫抗性进化对利用 Bt 杀虫 Cry 毒素的生物农药和转基因作物的优势构成了重大威胁。然而，关于鳞翅目昆虫特定毒素受体的转录调控与其对 Bt 毒素抗性之间关系的研究有限。在这里，我们报告了 SfGATAe 转录因子对 ABCC2 基因表达的正调控作用。利用 DAP-seq 技术和启动子缺失作图，启动子中对 SfGATAe 调控至关重要的 DNA 区域。通过酵母单杂交实验、DNA Pull-down实验和定点突变，我们证实转录因子SfGATAe对靶基因中的核心控制位点PBS2进行调控。组织特异性表达分析表明，SfGATAe 参与秋粘虫中肠细胞的调节和表达。草地贪夜蛾幼虫的沉默会导致 Cry1Ac 毒素的表达减少和敏感性降低。总体而言，本研究阐明了转录因子SfGATAe对毒素受体基因表达的调控机制，以及这种转录控制机制影响草地贪夜蛾对Bt毒素的抗性。