Vascular dementia (VD), a chronic syndrome of acquired intellectual impairment resulting from cerebrovascular diseases, is closely linked to neuronal autophagy and apoptosis. As a standardized extract from L. leaves, EGb761 is widely applied for treating cerebrovascular diseases owing to its neuroprotective effects, with on-going research exploring its therapeutic mechanisms. Rat model of VD and SH-SY5Y cell model with OGD/R injury was applied for this study. Results showed that EGb761 reduces OGD/R-elicited apoptosis and autophagosome production, whereas improving the viability of hippocampal neurons. EGb761 treatment led to a decrease in the ratios of p-AMPK/AMPK, Bax/Bcl-2, cleaved caspase-3/caspase-3, cleaved caspase-9/caspase-9, LC3-II/LC3-I, and level of Beciln-1, as well as enhancing the proportion of p-mTOR/mTOR. Additionally, EGb761 indicated a protective effect on rats' cognitive function. These results demonstrated EGb761′s specific therapeutic mechanism in preventing apoptosis and autophagy in VD models and improving cognitive functioning in VD rats through AMPK-mTOR signalling.

中文翻译：

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银杏叶提取物 (EGb761) 通过 AMPK-mTOR 信号通路抑制血管性痴呆大鼠模型的自噬和凋亡


血管性痴呆（VD）是一种由脑血管疾病引起的获得性智力障碍的慢性综合征，与神经元自噬和凋亡密切相关。 EGb761 作为 L. 叶的标准化提取物，由于其神经保护作用而被广泛应用于治疗脑血管疾病，并且正在进行研究探索其治疗机制。本研究采用VD大鼠模型和OGD/R损伤的SH-SY5Y细胞模型。结果表明，EGb761 减少 OGD/R 引发的细胞凋亡和自噬体产生，同时提高海马神经元的活力。 EGb761处理导致p-AMPK/AMPK、Bax/Bcl-2、cleaved caspase-3/caspase-3、cleaved caspase-9/caspase-9、LC3-II/LC3-I的比率和水平降低Beciln-1 的含量，以及提高 p-mTOR/mTOR 的比例。此外，EGb761 对大鼠的认知功能具有保护作用。这些结果证明了EGb761在VD模型中预防细胞凋亡和自噬以及通过AMPK-mTOR信号传导改善VD大鼠的认知功能的特异性治疗机制。