Drought, as a natural disaster of force majeure, significantly disrupts the physiological balance of plants and crop yields. The basic leucine zipper (bZIP) transcription factors assume the pivotal role of paramount regulators in orchestrating drought response. In this study, a bZIP transcription factor was isolated on the base of transcriptome, and its function in response to drought stress was verified. had a 579 bp open reading frame, encoded 192 amino acids, and existed in a conserved structural domain typical of bZIP, and was highly expressed when was stressed by drought. Moreover, the subcellular localization of PobZIP4 was observed with fluorescent protein labeling method and it was found that PobZIP4 localized in the epidermal cell nucleus. Then, we silenced in using virus-induced gene silencing technology, and found that -silenced plants showed significantly less tolerant to drought stress than the negative control. The accumulation of reactive oxygen species was found to be heightened, while the photosynthetic capacity, proline content, and protective enzyme activities exhibited a significant decline upon silencing , which unequivocally demonstrated that amplifies the drought tolerance of . This study highlighted the positive role of in mitigating drought stress in , thereby establishing a molecular basis for enhancing the drought tolerance of .

中文翻译：

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牡丹干旱相关PobZIP4转录因子的分离及功能分析

干旱作为不可抗力的自然灾害，严重破坏植物生理平衡和农作物产量。基本亮氨酸拉链（bZIP）转录因子在协调干旱反应中发挥着至关重要的调节作用。本研究在转录组的基础上分离出了bZIP转录因子，并验证了其响应干旱胁迫的功能。具有579 bp的开放阅读框，编码192个氨基酸，存在于bZIP典型的保守结构域中，并且在干旱胁迫时高表达。此外，通过荧光蛋白标记法观察PobZIP4的亚细胞定位，发现PobZIP4定位于表皮细胞核。然后，我们利用病毒诱导的基因沉默技术进行沉默，发现沉默的植物对干旱胁迫的耐受性明显低于阴性对照。发现沉默后活性氧的积累增加，而光合能力、脯氨酸含量和保护酶活性显着下降，这明确表明增强了 的耐旱性。本研究强调了其在缓解干旱胁迫方面的积极作用，从而为增强其耐旱性奠定了分子基础。