The use of TDM in clinical practice to monitor the plasma levels of antibiotics administered to critically ill patients is a well-established approach that allows for optimization of the patient's response to drug therapy, considering the characteristics of the drug, the clinical and physiological status of the patient and any peculiar of the pathogen that caused the clinical picture. In our laboratory, we have developed a single LC-MS/MS analysis for dosing the serum concentration of an antibacterial panel composed of eight antibacterial and two selective inhibitors. The method presented used a certified material furnished by a commercial company and was internally validated using the EMA guidelines. The results have shown high sensitivity, precision, and accuracy, a lower matrix effect combined with simple sample preparation and a time-saving procedure. We have evaluated the recovery rate and matrix effect by testing serum samples without pathological index and serum pools obtained from haemolysed, icteric, or lipemic samples. The assay has shown a recovery range between 94% and 101%.

中文翻译：

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开发同时定量人血清中十种常用抗生素药物的快速 LC-MS/MS 方法

在临床实践中使用 TDM 来监测危重患者服用的抗生素血浆水平是一种行之有效的方法，考虑到药物的特性、患者的临床和生理状态，可以优化患者对药物治疗的反应。患者以及引起临床表现的任何特殊病原体。在我们的实验室中，我们开发了一种单一的 LC-MS/MS 分析方法，用于测定由八种抗菌剂和两种选择性抑制剂组成的抗菌组的血清浓度。该方法使用了商业公司提供的经过认证的材料，并使用 EMA 指南进行了内部验证。结果显示出高灵敏度、精密度和准确度、较低的基质效应以及简单的样品制备和节省时间的程序。我们通过测试无病理指标的血清样品和从溶血、黄疸或血脂样品中获得的血清池来评估回收率和基质效应。该测定显示回收率在 94% 至 101% 之间。