Finding safe sprout inhibitors to mitigate premature sprouting of potato tubers during postharvest storage is critical for preserving tuber quality and marketability within the potato industry. Investigating the impact of promising sprout inhibitor treatments on molecular mechanisms and metabolic pathways that regulate tuber dormancy has wider implications, especially to find relevant biomarkers and to identify their modes of action. In this study, dormant cv. Russet Burbank tubers were treated with 1,4-dimethylnaphthalene (DMN) and methyl jasmonate (MeJa) alone or in combination to determine their effects on sprout growth. Changes in expression of genes involved in phytohormonal pathways, cell cycle, and dormancy-related processes were determined 0–21 days after treatments. Biochemical regulation associated with carbohydrate metabolism and plant stress responses were also determined. Significant sprout suppression was observed with MeJa and DMN+MeJa treatments during long-term storage. These sprout inhibitor treatments also resulted in increased abundance of transcripts associated with abscisic acid, brassinosteroids, and dormancy regulation. While transcript abundance of cytokinin and select cell cycle genes decreased, especially with DMN treatment, lower metabolic activity related to carbohydrate metabolism was observed in bud meristem tissues when compared to tuber flesh. Overall, MeJa enhanced stress protective metabolites such as phenolic acids and increased antioxidant enzyme responses in bud meristem tissues. Collectively, results of this study indicate a stress inducive mode of action of MeJa, which might have played a role in sprout suppression during long-term storage of potato tubers.

中文翻译：

---

茉莉酸甲酯和1,4-二甲基萘对马铃薯块茎休眠中植物激素和应激保护途径调节的影响不同

寻找安全的发芽抑制剂来减轻马铃薯块茎在采后储存期间的过早发芽对于保持马铃薯行业内的块茎质量和适销性至关重要。研究有前途的芽抑制剂治疗对调节块茎休眠的分子机制和代谢途径的影响具有更广泛的意义，特别是寻找相关的生物标志物并确定其作用模式。在这项研究中，休眠简历。赤褐色伯班克块茎用 1,4-二甲基萘 (DMN) 和茉莉酸甲酯 (MeJa) 单独或组合处理，以确定它们对芽生长的影响。治疗后 0-21 天测定涉及植物激素途径、细胞周期和休眠相关过程的基因表达的变化。还确定了与碳水化合物代谢和植物应激反应相关的生化调节。在长期储存期间，MeJa 和 DMN+MeJa 处理观察到显着的发芽抑制。这些发芽抑制剂治疗还导致与脱落酸、油菜素类固醇和休眠调节相关的转录物丰度增加。虽然细胞分裂素和选择细胞周期基因的转录丰度下降，特别是在DMN处理后，但与块茎果肉相比，在芽分生组织中观察到与碳水化合物代谢相关的代谢活性较低。总体而言，MeJa 增强了芽分生组织中的应激保护性代谢物（例如酚酸）并增加了抗氧化酶反应。总的来说，这项研究的结果表明了 MeJa 的胁迫诱导作用模式，这可能在马铃薯块茎长期储存期间的发芽抑制中发挥了作用。