The global presence of SARS-CoV-2 in household pets is acknowledged, yet documentation remains scarce, leaving many regions unexplored. Thus, our study sought to fill this gap by investigating SARS-CoV-2 presence in dogs visiting veterinary clinics during the third pandemic peak in eastern Colombia. We collected and analyzed 43 oropharyngeal and rectal swabs using real-time PCR assays targeting the Envelope Gene of SARS-CoV-2. Out of these, two dogs tested positive, indicating an infection rate of 4.7%. Further examination through complete sequencing and phylogenetic analysis revealed the lineage B.1.621 for the SARS-CoV-2 genome. Consequently, our study unveils the first documented cases of Canis lupus familiaris infected with the Mu variant of SARS‐CoV‐2, the variant with the most death burden during the whole pandemic in Colombia. Remarkably, these cases presented mild and reversible respiratory and gastrointestinal symptoms, or no clinical manifestations at all. This sheds light on the virus's interaction with our four-legged companions, offering valuable insights into its transmission dynamics and potential effects on animal health.

全球范围内的家庭宠物中存在 SARS-CoV-2，这一点已得到公认，但相关文献仍然很少，导致许多地区尚未被探索。因此，我们的研究试图通过调查哥伦比亚东部第三次大流行高峰期间前往兽医诊所的狗中是否存在 SARS-CoV-2 来填补这一空白。我们使用针对 SARS-CoV-2 包膜基因的实时 PCR 检测收集并分析了 43 份口咽和直肠拭子。其中，有两只狗检测呈阳性，表明感染率为4.7%。通过完整测序和系统发育分析的进一步检查揭示了 SARS-CoV-2 基因组的谱系 B.1.621。因此，我们的研究揭示了第一例记录在案的家犬感染 SARS-CoV-2 Mu 变种的病例，该变种在哥伦比亚整个大流行期间死亡负担最大。值得注意的是，这些病例表现出轻微且可逆的呼吸道和胃肠道症状，或者根本没有临床表现。这揭示了病毒与我们的四足同伴的相互作用，为了解其传播动态和对动物健康的潜在影响提供了宝贵的见解。