NARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is part of the catalytic triad. Here we report that unlike suggested previously, H234 instead of H233 is a component of the catalytic triad alongside residues D291 and S385 in NAL1. Remarkably, residue 233 unexpectedly plays a pivotal role in regulating NAL1’s proteolytic activity. These findings establish a strong foundation for utilizing NAL1 in breeding programs aimed at improving crop yield.

中文翻译：

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水稻 NARROW LEAF1 的催化三联体涉及 H234

NARROW LEAF1 ( NAL1 ) 对叶片形态和作物产量产生多方面的影响。最近的晶体研究表明组氨酸 233 (H233) 是催化三联体的一部分。在这里，我们报告，与之前建议的不同，H234 而不是 H233 与 NAL1 中的残基 D291 和 S385 一起成为催化三联体的组成部分。值得注意的是，残基 233 出人意料地在调节 NAL1 的蛋白水解活性中发挥着关键作用。这些发现为在旨在提高作物产量的育种计划中利用 NAL1 奠定了坚实的基础。